Regio- and stereospecific analysis of glycerolipids
Regio- and stereospecific analysis of glycerolipids
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DOI：
10.1016/j.ymeth.2004.11.001
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发表时间：
2005-06-01
期刊：
影响因子：
4.8
通讯作者：
Itabashi, Y
中科院分区：
文献类型：
--
作者：
Kuksis, A;Itabashi, Y
近年来，研究人员已经认识到综合脂质分析（脂质组学）的潜在价值，这是由脂质学家发明和推广的，他们认识到DNA分析（基因组学）和蛋白质分析（蛋白质组学）领域的许多有价值的应用。通过脂质类选择性内电离和随后的二维交叉峰强度的分析，大多数脂质类的单个分子种类的化学身份和质量组成现在可以在氯仿提取物中确定。然而，仍然有必要区分由酶和化学反应产生的对映异构体和同量异位区域异构体，常规高效液相色谱/质谱法（HPLC/MS）已经缓慢适应，并且串联MS不能提供。虽然反相HPLC可以分离区域异构体，正相HPLC可以拆分非对映异构体，手性HPLC可以实现对映异构体的显著拆分，但组合系统的全部潜力很少被开发，本章呼吁注意最近和早期这些方法与质谱的组合，其允许HPLC/ESI（电喷雾电离）-MS/MS分离和鉴定对映体二酰基甘油、三酰基甘油。和甘油磷脂以及它们的同量异位区域异构体。这些发展允许进一步扩展脂质谱（脂质组学）和更好地了解脂质代谢。(c)2005爱思唯尔公司All rights reserved.
In recent years researchers have recognized the potential value of comprehensive lipid profiling (lipidomics), which was invented and promoted by lipidologists who recognized the many Valuable applications that grew out of the fields of DNA profiling (genomics) and protein profiling (proteonomics). Through lipid class-selective intrasource ionization and subsequent analysis of two-dimensional cross-peak intensities, the chemical identity and mass composition of individual molecular species of most lipid classes can now be determined in a chloroform extract. There remains, however, the necessity to distinguish the enantiomers and isobaric regioisomers resulting from enzymatic and chemical reactions, which conventional high performance liquid chromatography/mass spectrometry (HPLC/MS) has been slow to accommodate, and tandem MS unable to provide. While reversed-phase HPLC can separate regioisomers, normal-phase HPLC can resolve diastereomers, and chiral-phase HPLC can effect dramatic resolution of enantiomers, the full potential of the combined systems has seldom been exploited, The present chapter calls attention to both recent and earlier combinations of these methodologies with mass spectrometry, which allows the HPLC/ESI (electrospray ionization)-MS/MS separation and identification of enantiomeric diacylglycerols, triacylglycerols. and glycerophospholipids as well as their isobaric regioisomers. These developments permit further expansion of lipid profiling (lipidomics) and better understanding of lipid metabolism. (c) 2005 Elsevier Inc. All rights reserved.