High-resolution MIRU-VNTRs typing reveals the unique nature of Mycobacterium tuberculosis Beijing genotype in Okinawa, Japan
High-resolution MIRU-VNTRs typing reveals the unique nature of Mycobacterium tuberculosis Beijing genotype in Okinawa, Japan
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DOI：
10.1016/j.meegid.2011.06.013
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发表时间：
2012-06-01
影响因子：
3.2
通讯作者：
Rastogi, Nalin
中科院分区：
文献类型：
--
作者：
Millet, Julie;Miyagi-Shiohira, Chika;Rastogi, Nalin
结核分枝杆菌北京系在日本高度流行。本研究的目的是基于12、15和21位点的MIRU-VNTR基因分型方案来描述中国北京世系的种群结构。将来自琉球群岛冲绳的北京毒株的MIRU-VNTR模式与最近公布的来自日本本州主岛大阪-神户特大城市的MIRU-VNTR模式进行了比较（Wada et al., 2009）。我们还比较了“现代/典型”与“典型”的比例。根据NTF位点结构定义的“古/非典型”北京菌株。与12位点的最小生成树（MST）相反，15位点和21位点的树可以区分冲绳的两组菌株。与MIRU国际型MIT17相对应的12个位点的MIRU- vntr型（223325173533）是日本最普遍的北京基因型。在SITVIT2数据库中，发现这种模式在世界范围内传播，并与东亚和前苏联国家中分布最广泛的北京概况相对应。15位点和21位点的MIRU-VNTR模式比较表明，两个位点（QUB-4156和Mtub21）在我们的研究中多态性最多，可能是区分北京菌株NTF位点亚分类的潜在候选位点。利用15个和21个位点的高分辨率VNTR分型强调了冲绳北京基因型的“古代/非典型”亚群，以及与大阪/神户观察到的“现代/典型”北京菌株密切相关的菌株亚群。(C) 2011 Elsevier B.V.版权所有
Mycobacterium tuberculosis Beijing lineage is highly prevalent in Japan. The aim of the present study was to describe the population structure of the Beijing lineage in this country based on 12-, 15-, and 21-loci MIRU-VNTR genotyping schemes. The MIRU-VNTR patterns of Beijing strains from Okinawa, Ryukyu Islands were compared to those recently published from the Osaka-Kobe megalopolis of the main island of Japan, Honshu (Wada et al., 2009). We also compared proportions of "modern/typical" vs. "ancient/atypical" Beijing strains as defined by structure of the NTF locus. Contrarily to the 12-loci Minimum Spanning Tree (MST), the 15- and 21-loci trees allowed the distinction of two groups of strains in Okinawa. A 12-loci MIRU-VNTR pattern (223325173533) corresponding to MIRU international type MIT17 was identified as the most prevalent Beijing genotype in Japan. In the SITVIT2 database, this pattern was found to be disseminated worldwide and corresponded to the most widely distributed Beijing profile in East Asia and former USSR countries. A comparison of 15- and 21-loci MIRU-VNTR patterns showed that two loci (QUB-4156 and Mtub21) were most polymorphic in our study, and could be potential candidates to distinguish between NTF locus based subclassification of Beijing strains. High-resolution VNTR typing using 15- and 21-loci underlined an evolutionarily distinct "ancient/atypical" subpopulation of the Beijing genotype in Okinawa as well as a subgroup of strains closely related to "modern/typical" Beijing strains observed in Osaka/Kobe. (C) 2011 Elsevier B.V. All rights reserved.