Establishment of an induced pluripotent stem cell line from a female domestic ferret (Mustela putorius furo) with an X chromosome instability
Establishment of an induced pluripotent stem cell line from a female domestic ferret (Mustela putorius furo) with an X chromosome instability
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从具有 X 染色体不稳定性的雌性家养雪貂 (Mustela putorius Furo) 中建立诱导多能干细胞系
DOI：
10.1016/j.scr.2021.102385
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发表时间：
2021
影响因子：
1.2
通讯作者：
Okano Hideyuki
中科院分区：
文献类型：
--
作者：
Yoshimatsu Sho;Murakami Rei;Nakajima Mayutaka;Sato Tsukika;Kawasaki Hiroshi;Okano Hideyuki
国内雪貂（雪貂;由于其高度发达的大脑皮层和对禽流感和冠状病毒的易感性，Mustela putorius furo是神经科学和临床前/兽医学的重要动物模型。然而，由于包括诱导多能干细胞（iPSC）在内的雪貂永生细胞系一直稀缺，因此缺乏体外雪貂模型。在这项研究中，我们从雪貂皮肤成纤维细胞建立了iPSC系。所建立的iPSC系，fiPS-1，显示多能性的标准特征，但其X染色体不稳定。总之，本研究为雪貂的体外模型研究提供了有用的资料。
The domestic ferret (ferret;Mustela putorius furo) is an important animal model for neuroscience and preclinical/veterinary medicine owing to its highly developed cerebral cortex and susceptibility to avian influenza and corona viruses. Nevertheless, there is a lack ofin vitroferret models, since immortal cell lines including induced pluripotent stem cells (iPSCs) of ferrets have been scarce. In this study, we established an iPSC line from ferret skin fibroblasts. The established iPSC line, fiPS-1, showed standard characteristics of pluripotency, but its X chromosome was unstable. Collectively, the present study provides a useful resource forin vitromodel using the ferret.