Cell wall metabolism in ripening fruit .9. Synthesis of pectic and hemicellulosic cell wall polymers in the outer pericarp of mature green tomatoes (cv XMT-22)
Cell wall metabolism in ripening fruit .9. Synthesis of pectic and hemicellulosic cell wall polymers in the outer pericarp of mature green tomatoes (cv XMT-22)
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DOI：
10.1104/pp.114.4.1523
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发表时间：
1997-08-01
期刊：
影响因子：
7.4
通讯作者：
Labavitch, JM
中科院分区：
文献类型：
--
作者：
Huysamer, M;Greve, LC;Labavitch, JM
从成熟的绿色番茄（Lycopersicon esculentum Mill.）果实中切下外果皮圆盘，并在无菌组织培养板中保存4天，包括用D-[U-C-13]葡萄糖孵育2天。制备细胞壁并提取水溶性、果胶和半纤维素聚合物。通过重同位素标记掺入的气相色谱-质谱分析测定细胞壁合成能力。根据中性糖标记的百分比，包括角质层的“外部”2 毫米果皮区域的细胞壁合成能力低于其正下方（更靠近小室）的“内部”2 毫米区域。两个组织区域中糖苷键的相对丰度没有显着差异。将标签掺入中性糖中，并在细胞壁制剂中鉴定出每种多糖类的典型连接。半乳糖醛酸和葡萄糖醛酸的标记程度与每个组织区域中中性糖的标记程度相似。
Discs of outer pericarp were excised from mature green tomato (Lycopersicon esculentum Mill.) fruit and kept in sterile tissue culture plates for 4 d, including 2 d of incubation with D-[U-C-13]glucose. Cell walls were prepared and the water-soluble, pectic, and hemicellulosic polymers were extracted. Cell wall synthetic capacity was determined by gas chromatography-mass spectrometry analysis of incorporation of the heavy isotope label. The ''outer'' 2-mm pericarp region, which included the cuticle, had a lower cell wall synthetic capacity than the ''inner'' 2-mm region immediately below it (closer to the locules), based on the percentage of labeling of the neutral sugars. There were no significant differences in relative abundance of glycosidic linkages in the two tissue regions. Label was incorporated into neutral sugars and linkages typical for each polysaccharide class were identified in the cell wall preparations. Galacturonic acid and glucuronic acid were labeled to an extent similar to that of the neutral sugars in each tissue region.