Photoresponsive DNA monolayer prepared by primer extension reaction on the electrode
Photoresponsive DNA monolayer prepared by primer extension reaction on the electrode
复制标题
电极上引物延伸反应制备光响应DNA单层
作者：
Takada;T.; Takemura;M.; Kawano;Y.; Nakamura;M.; Yamana;K.
我们描述了一种简单方便的方法，用于制备光响应的DNA修饰电极使用引物延伸（PEX）反应。以萘酰亚胺衍生物为光敏剂，将其连接到2′-脱氧尿苷-5 ′-三磷酸（dUTPNI）的C5位上。已经发现dUTPNI是在溶液中使用KOD Dash和Vent（外切）酶将萘酰亚胺（NI）部分掺入DNA序列中的PEX反应的良好底物。在固定有引物/模板DNA的电极表面上，发现将dUTPNI分子掺入DNA序列中的PEX反应有效地进行。通过这种固相方法，可以构建能够因光响应NI分子而产生光电流的DNA单层。结果表明，光电流的产生显着抑制由引物/模板DNA中包括的单核苷酸错配，这是适用于光电化学传感器的设计，以区分单核苷酸序列。
We describe a simple and convenient method for the preparation of photoresponsive DNA-modified electrodes using primer extension (PEX) reactions. A naphthalimide derivative was used as the photosensitizer that was attached to the C5-position of 2′-deoxyuridine-5′-triphosphate (dUTPNI). It has been found that dUTPNIis a good substrate for the PEX reactions using KOD Dash and Vent (exo-) enzymes in solutions to incorporate naphthalimide (NI) moieties into the DNA sequences. On the electrode surface immobilized with the primer/template DNA, the PEX reactions to incorporate dUTPNImolecules into the DNA sequence were found to efficiently proceed. With this solid-phase method, the DNA monolayers capable of generating photocurrent due to the photoresponsive NI molecule can be constructed. It was shown that the photocurrent generation was significantly suppressed by a single-nucleotide mismatch included in the primer/template DNA, which is applicable for the design of photoelectrochemical sensors to discriminate single-nucleotide sequences.