SITE SPECIFIC INSERTION OF A TYPE-I RDNA ELEMENT INTO A UNIQUE SEQUENCE IN THE DROSOPHILA-MELANOGASTER GENOME
SITE SPECIFIC INSERTION OF A TYPE-I RDNA ELEMENT INTO A UNIQUE SEQUENCE IN THE DROSOPHILA-MELANOGASTER GENOME
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DOI：
10.1093/nar/12.23.9111
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发表时间：
1984-01-01
影响因子：
14.9
通讯作者：
GLOVER, DM
中科院分区：
文献类型：
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作者：
BROWNE, MJ;READ, CA;GLOVER, DM
我们描述了来自黑腹果蝇俄勒冈 R 品系的独特 DNA 克隆片段，其中包含主要在 rDNA 中发现的短 I 型插入片段。主要的 I 型 rDNA 插入长度为 5kb，但也有较短的 I 型元件的共端子集，它们与 rDNA 共享一个共同的右侧连接点。我们现在描述的插入是这个子集的另一个成员。 I型序列与独特DNA的右侧连接与I型序列与rDNA的右侧连接相同。插入序列中没有任何显着特征可以确定左连接与其插入序列的位置。与 rDNA 中相应的短 I 型插入一样，独特 DNA 中的插入两侧都有重复序列，在本例中长度为 10 个碱基对。通过观察 D. Karsnas 菌株，促进了对应于不间断的独特位置的序列的克隆。 Melanoqaster 仅包含此类不间断序列。插入目标位点处的重复序列仅以单个拷贝的形式存在于不间断的 DNA 中。独特片段中插入的靶位点序列 (ACTGTTCT) 显示出与 rDNA 中的靶标 (ACTGTCCC) 具有惊人的同源性。
We describe a cloned segment of unique DNA from the Oregon R strain of Drosophila melanogaster that contains a short type I insertion of the kind principally found within rDNA. The predominant type I rDNA insertion is 5kb in length, but there are also a co-terminal sub-set of shorter type I elements that share a common right hand junction with the rDNA. The insertion that we now describe is another member of this sub-set. The right hand junction of the type I sequence with the unique DNA is identical to the right hand junction of the type I sequences with rDNA. There is no significant feature within the insertion sequence that could have determined the position of the left junction with the sequence into which it is inserted. Like the corresponding short type I insertions in rDNA, the insertion into the unique DNA is flanked on both sides by a duplicated sequence, which in this case is 10 base pairs long. The cloning of a sequence corresponding to the uninterrupted unique location was facilitated by the observation that the Karsnas strain ofD. melanoqastercontains only uninterrupted sequences of this kind. The duplicated sequence at the target site for the insertion is only present as a single copy in the uninterrupted DNA. The sequence of the target site for the insertion (ACTGTTCT) in the unique segment shows a striking homology to the target in rDNA (ACTGTCCC).