Hyaluronic acid secretion by synoviocytes alters under cyclic compressive load in contracted collagen gels
Hyaluronic acid secretion by synoviocytes alters under cyclic compressive load in contracted collagen gels
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滑膜细胞的透明质酸分泌在收缩的胶原凝胶中的循环压缩载荷下发生变化
DOI：
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
Y. Nomura
中科院分区：
文献类型：
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作者：
K. Uehara;M. Hara;Toshiki Matsuo;Go Namiki;M. Watanabe;Y. Nomura
膝关节骨性关节炎是一种退行性关节疾病。生物力学因素被认为是该疾病的危险因素，膝关节通常受到压力。一些研究已经在体外检查了膝关节的生物力学环境。本研究的目的是建立一个模拟膝关节环境的培养模型。作为第一步，滑膜细胞诱导三维胶原凝胶收缩。接下来，使用FX-4000 C ™ Flexercell® Compression Plus™系统，将含有滑膜细胞的收缩胶原凝胶以1.0 Hz的频率在0至40 kPa范围内循环压缩1.5、3、6和12 h。胶原凝胶中的RNA在压缩后立即提取，并通过定量RT-PCR分析HAS基因的mRNA表达水平。压缩后48小时收集培养基，并通过琼脂糖凝胶电泳和醋酸纤维素电泳进行分析。收缩胶原凝胶中的滑膜细胞受到循环压缩负荷的刺激。长期的压缩刺激导致产生更高分子量的透明质酸，而短期的压缩刺激增加了透明质酸的总量。此外，HAS-1和HAS-2的mRNA表达水平均显著高于未压缩的情况。总之，使用该凝胶培养系统，滑膜细胞合成更高分子量的透明质酸，并通过上调HAS基因表达产生大量透明质酸。因此，收缩的胶原凝胶模型将是一个有用的体外三维模型的膝关节。
Knee osteoarthritis is a degenerative disease of diarthrodial joints. Biomechanical factors are considered as risk factors for the disease, the knee joint being normally subject to pressure. Some studies have examined the biomechanical environment of the knee joint in vitro. The aim of this study was to establish a culture model to mimic the knee joint environment. As a first step, synoviocytes induced contraction of three-dimensional collagen gels. Next, contracted collagen gels containing synoviocytes underwent cyclical compression ranging from 0 to 40 kPa at a frequency of 1.0 Hz for 1.5, 3, 6 and 12 h using the FX-4000C™ Flexercell® Compression Plus™ System. RNA in collagen gels was extracted immediately after compression and mRNA expression levels of HAS genes were analyzed by quantitative RT-PCR. Culture medium was collected 48 h after compression and analyzed by agarose gel electrophoresis and cellulose acetate electrophoresis. Synoviocytes in contracted collagen gels were stimulated by cyclic compressive load. Long-term compressive stimulation led to the production of higher molecular weight hyaluronic acid, whereas, short-term, compressive stimulation increased the total amount of hyaluronic acid. Furthermore, mRNA expression levels of both HAS-1 and HAS-2 were significantly higher than without compression. Taken together, using this gel culture system, synoviocytes synthesized higher molecular weight hyaluronic acid and produced large quantities of hyaluronic acid through up-regulation of HAS gene expression. Therefore, the contracted collagen gel model will be a useful in vitro three-dimensional model of the knee joint.
机械压缩调节软骨细胞/琼脂糖培养物中的基质生物合成。
影响因子：
4
作者：
Buschmann,MD;Gluzband,YA;Grodzinsky,AJ;Hunziker,EB
通讯作者：
Hunziker,EB