Biomembrane disruption by silica-core nanoparticles: effect of surface functional group measured using a tethered bilayer lipid membrane.
Biomembrane disruption by silica-core nanoparticles: effect of surface functional group measured using a tethered bilayer lipid membrane.
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DOI：
10.1016/j.bbamem.2013.09.007
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发表时间：
2014-01
影响因子：
3.4
通讯作者：
Worden, R. Mark
中科院分区：
文献类型：
--
作者：
Liu, Ying;Zhang, Zhen;Zhang, Quanxuan;Baker, Gregory L.;Worden, R. Mark
工程纳米材料（ENM）具有令人满意的特性，使其非常适合许多商业应用。然而，对ENM的性质如何影响其与生物膜的分子相互作用的有限理解阻碍了设计安全有效的ENM的努力。本文介绍了使用拴系双层脂质膜（tBLM）的功能化的二氧化硅核纳米粒子的生物膜破坏的特点。电化学阻抗谱用于测量纳米颗粒暴露后tBLM电阻的时间轨迹。然后使用来自指数电阻衰减模型的参数的统计分析来量化和分析未官能化、胺官能化或羧基官能化的纳米颗粒的阻抗曲线之间的差异。所有的纳米颗粒引发膜电阻的降低，表明纳米颗粒诱导的tBLM的破坏。分级聚类允许纳米颗粒降低tBLM抗性的效力以胺>羧基~裸二氧化硅的顺序排列。动态光散射分析表明，tBLM曝光引发轻微的合并为裸和胺官能化的二氧化硅纳米粒子，但不为羧基官能化的二氧化硅纳米粒子。这些结果表明，tBLM方法可以再现表征ENM诱导的生物膜破坏，并可以区分除了其表面官能团之外相同的纳米颗粒的BLM破坏模式。该方法提供了对涉及生物膜的分子相互作用机制的深入了解，并且适用于高通量应用的小型化和自动化，以帮助评估纳米材料暴露的健康风险或识别具有与生物膜相互作用的期望模式的ENM。
Engineered nanomaterials (ENM) have desirable properties that make them well suited for many commercial applications. However, a limited understanding of how ENM’s properties influence their molecular interactions with biomembranes hampers efforts to design ENM that are both safe and effective. This paper describes the use of a tethered bilayer lipid membrane (tBLM) to characterize biomembrane disruption by functionalized silica-core nanoparticles. Electrochemical impedance spectroscopy was used to measure the time trajectory of tBLM resistance following nanoparticle exposure. Statistical analysis of parameters from an exponential resistance decay model was then used to quantify and analyze differences between the impedance profiles of nanoparticles that were unfunctionalized, amine-functionalized, or carboxyl-functionalized. All of the nanoparticles triggered a decrease in membrane resistance, indicating nanoparticle-induced disruption of the tBLM. Hierarchical clustering allowed the potency of nanoparticles for reducing tBLM resistance to be ranked in the order amine > carboxyl ~ bare silica. Dynamic light scattering analysis revealed that tBLM exposure triggered minor coalescence for bare and amine-functionalized silica nanoparticles but not for carboxyl-functionalized silica nanoparticles. These results indicate that the tBLM method can reproducibly characterize ENM-induced biomembrane disruption and can distinguish the BLM-disruption patterns of nanoparticles that are identical except for their surface functional groups. The method provides insight into mechanisms of molecular interaction involving biomembranes and is suitable for miniaturization and automation for high-throughput applications to help assess the health risk of nanomaterial exposure or identify ENM having a desired mode of interaction with biomembranes.
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DOI：
10.1098/rspa.1937.0109
发表时间：
1937-05-01
影响因子：
--
作者：
Bartlett, MS
通讯作者：
Bartlett, MS
影响因子：
15
作者：
Hicks, JF;Miles, DT;Murray, RW
通讯作者：
Murray, RW
影响因子：
12.6
作者：
Jadhav, Sachin R.;Zheng, Yi;Worden, R. Mark
通讯作者：
Worden, R. Mark
影响因子：
9.9
作者：
Jadhav, Sachin R.;Rao, Kota Sreenivasa;Worden, R. Mark
通讯作者：
Worden, R. Mark
影响因子：
3.9
作者：
Kang, Jing;Rowntree, Paul A.
通讯作者：
Rowntree, Paul A.