Dopaminergic neurons encode a distributed, asymmetric representation of temperature in Drosophila.
Dopaminergic neurons encode a distributed, asymmetric representation of temperature in Drosophila.
复制标题
DOI：
10.1523/jneurosci.3933-12.2013
复制
发表时间：
2013-01-30
期刊：
影响因子：
--
通讯作者：
Tomchik SM
中科院分区：
文献类型：
--
作者：
Tomchik SM
多巴胺能回路调节动物的各种先天和习得行为，包括嗅觉联想学习、唤醒和温度偏好行为。目前尚不清楚是否不同或重叠的多巴胺能神经元调节这些行为。在这里，我有功能特点的多巴胺能电路支配果蝇蘑菇体在体内钙成像和遗传定义的神经元子集的条件性沉默。不同子集的PPL 1多巴胺能神经元支配的垂直叶的蘑菇体响应温度下降，但不增加，迅速适应爆发的活动。支配水平叶的PAM神经元对温度变化没有反应。消融的触角和下颚须减少，但没有消除，反应。遗传沉默的多巴胺能神经元支配的垂直蘑菇体叶大幅减少行为避冷，但沉默这些神经元的小子集没有影响。这些数据表明，重叠的多巴胺能电路编码广泛分布的，不对称的温度表示，覆盖区域先前涉及学习，记忆和遗忘。因此，不同的行为涉及重叠的多巴胺能神经元，编码多模式刺激和神经支配一个单一的解剖目标，蘑菇体。
Dopaminergic circuits modulate a wide variety of innate and learned behaviors in animals, including olfactory associative learning, arousal, and temperature preference behavior. It is not known whether distinct or overlapping sets of dopaminergic neurons modulate these behaviors. Here I have functionally characterized the dopaminergic circuits innervating the Drosophila mushroom body with in vivo calcium imaging and conditional silencing of genetically defined subsets of neurons. Distinct subsets of PPL1 dopaminergic neurons innervating the vertical lobes of the mushroom body responded to decreases in temperature, but not increases, with rapidly adapting bursts of activity. PAM neurons innervating the horizontal lobes did not respond to temperature shifts. Ablation of the antennae and maxillary palps reduced, but did not eliminate, the responses. Genetic silencing of dopaminergic neurons innervating the vertical mushroom body lobes substantially reduced behavioral cold avoidance, but silencing smaller subsets of these neurons had no effect. These data demonstrate that overlapping dopaminergic circuits encode a broadly distributed, asymmetric representation of temperature that overlays regions previously implicated in learning, memory, and forgetting. Thus, diverse behaviors engage overlapping sets of dopaminergic neurons that encode multimodal stimuli and innervate a single anatomical target, the mushroom body.