The Rab6-binding kinesin, Rab6-KIFL, is required for cytokinesis
The Rab6-binding kinesin, Rab6-KIFL, is required for cytokinesis
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DOI：
10.1093/emboj/19.21.5711
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发表时间：
2000-11-01
期刊：
影响因子：
11.4
通讯作者：
Barr, FA
中科院分区：
文献类型：
--
作者：
Hill, E;Clarke, N;Barr, FA
Rab 6结合驱动蛋白Rab 6-KIFL是在双杂交筛选中鉴定的与Rab 6相互作用的蛋白质，Rab 6是一种参与通过高尔基体的膜运输的小GTfls。我们发现Rab 6-KIFL在有丝分裂细胞中积累，在有丝分裂后期定位于纺锤体的中间区，在分裂末期定位于卵裂沟和中间体。Rab 6-KIFL的过表达引起细胞分裂缺陷，导致细胞死亡。显微注射Rab 6-KTFL抗体导致细胞在一个细胞周期后变成双核，延时显微镜显示这是由于卵裂沟形成缺陷，从而导致胞质分裂。这些数据表明，内源性Rab 6-KIFL在细胞分裂中的功能，在卵裂沟形成和胞质分裂，除了其先前描述的作用，在膜交通。
The Rab6-binding kinesin, Rab6-KIFL, was identified in a two-hybrid screen for proteins that interact with Rab6, a small GTPase involved in membrane traffic through the Golgi apparatus, We find that Rab6-KIFL accumulates in mitotic cells where it localizes to the midzone of the spindle during anaphase, and to the cleavage furrow and midbody during telophase. Overexpression of Rab6-KIFL causes a cell division defect resulting in cell death. Microinjection of antibodies to Rab6-KTFL results in the cells becoming binucleate after one cell cycle, and time-lapse microscopy reveals that this is due to a defect in cleavage furrow formation and thus cytokinesis. These data show that endogenous Rab6-KIFL functions in cell division during cleavage furrow formation and cytokinesis, in addition to its previously described role in membrane traffic.