Laser-illuminated nanohole arrays for multiplex plasmonic microarray sensing
Laser-illuminated nanohole arrays for multiplex plasmonic microarray sensing
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DOI：
10.1364/oe.16.000219
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发表时间：
2008-01-07
期刊：
影响因子：
3.8
通讯作者：
Oh, Sang-Hyun
中科院分区：
文献类型：
--
作者：
Lesuffleur, Antoine;Im, Hyungsoon;Oh, Sang-Hyun
表面等离子体共振（SPR）成像是一种强大的技术，用于高通量，实时，无标记的表征分子相互作用的微阵列格式。在本文中，我们展示了SPR成像与纳米孔阵列照明的激光源。周期性纳米孔将入射光子耦合到SP中，从而避免了对常规SPR仪器中使用的棱镜的需要，而激光源提供强度、稳定性和光谱相干性以提高检测灵敏度。在金上的烷硫醇盐的自组装单层的形成改变了超过35%的激光透射率，并且从纳米孔传感器的5 × 3微阵列平行地测量结合动力学。这些结果表明，纳米孔传感器的高通量SPR微阵列成像的潜力。(c)2008年美国光学学会。
Surface plasmon resonance (SPR) imaging is a powerful technique for high-throughput, real-time, label-free characterization of molecular interactions in a microarray format. In this paper, we demonstrate SPR imaging with nanohole arrays illuminated by a laser source. Periodic nanoholes couple incident photons into SPs, obviating the need for the prism used in conventional SPR instruments, while a laser source provides the intensity, stability and spectral coherence to improve the detection sensitivity. The formation of a self-assembled monolayer of alkanethiolates on gold changed the laser transmission by more than 35%, and binding kinetics were measured in parallel from a 5 x 3 microarray of nanohole sensors. These results demonstrate the potential of nanohole sensors for high-throughput SPR imaging on microarrays. (c) 2008 Optical Society of America.