Pig peripheral blood mononuclear leucocyte subsets are heritable and genetically correlated with performance
Pig peripheral blood mononuclear leucocyte subsets are heritable and genetically correlated with performance
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DOI：
10.1017/s1751731108002929
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发表时间：
2008-11-01
期刊：
影响因子：
3.6
通讯作者：
Bishop, S. C.
中科院分区：
文献类型：
--
作者：
Clapperton, M.;Glass, E. J.;Bishop, S. C.
用于选择猪以提高对感染的抵抗力或改善健康的指示性状必须是可遗传的，并且优选地与改善的性能相关。我们估计了一系列免疫性状的遗传力及其与生长性能的遗传和表型相关性。我们测量了来自6个农场的589头猪的免疫性状和1941头猪的性能，其中3头被归类为“高健康状态”（即无特定病原体），3头健康状态较低。所有的猪都很健康。免疫性状为标准生长性能试验开始和结束时的总白色血细胞（WBC）和外周血单核白细胞（PBML）亚群，其对CD 4、CD 8 α、γ δ（γ δ）T细胞受体、CD 11 R1（自然杀伤细胞标志物）、B细胞和单核细胞标志物呈阳性。在两个时间点，除CD 8 α（+）细胞外，所有免疫性状均具有中度至高度遗传性。在检验结束时，遗传力估计值（h（2））（+/- s.e.）总WBC计数为0.18（+/- 0.11）。对于PBML亚群比例，γ δ TCR细胞的遗传率为0.52（+/- 0.14），CD 4（+）细胞为0.62（+/- 0.14），CD 11 R1（+）细胞为0.44（+/- 0.14），B细胞为0.58（+/- 0.14），单核细胞为0.59（+/- 0.14）。农场健康状况影响WBC的遗传力，在健康状况较低的农场中显著较高，但对PBML亚群的遗传力没有一致的影响。在测试开始和结束时，各种PBML子集的数量和比例与性能之间存在显着的负遗传相关。特别是，在试验结束时，PBML细胞中CD 11 R1（+）细胞的比例与日增重密切相关（r（g）= -0.72; P < 0.01）。对于γ δ（+）T细胞、CD 8 α（+）、CD 11 R1（+）细胞、B细胞或单核细胞，试验结束时测量的PBML亚群与性能之间也存在较弱但显著的负表型相关性。与日增重的表型相关性在试验开始时一般低于试验结束时。这些结果表明，大多数主要的猪PBML亚群是遗传的，这些PBML亚群的几个系统水平与性能呈遗传负相关。这种方法提供了一个基础，使用免疫性状标记时，选择公猪，可以产生更高性能的后代。
Indicator traits used to select pigs for increased resistance to infection or improved health must be heritable and, preferably, be associated with improved performance. We estimated the heritability of a range of immune traits and their genetic and phenotypic correlations with growth performance. We measured immune traits on 589 pigs and performance on 1941 pigs from six farms, three of which were classified as 'high health status' (i.e. specific pathogen-free) and three were of lower health status. All pigs were apparently healthy. Immune traits were total white blood cells (WBC), and peripheral blood mononuclear leucocyte (PBML) subsets positive for CD4, CD8 alpha, gamma delta (gamma delta) T cell receptor, CD11R1 (natural killer cell marker), B cell and monocyte markers at the start and the end of standard growth performance tests. At both time points, all immune traits were moderately to highly heritable except for CD8 alpha(+) cells. At end of test, heritability estimates (h(2)) (+/- s.e.) were 0.18 (+/- 0.11) for total WBC count. For PBML subset proportions, the heritabilities were 0.52 (+/- 0.14) for gamma delta TCR cells, 0.62 (+/- 0.14) for CD4(+) cells, 0.44 (+/- 0.14) for CD11R1(+) cells, 0.58 (+/- 0.14) for B cells and 0.59 (+/- 0.14) for monocytes. Farm health status affected the heritabilities for WBC, being substantially higher on lower health status farms, but did not have consistent effects on heritabilities for the PBML subsets. There were significant negative genetic correlations between numbers and proportions of various PBML subsets and performance, at both start and end of test. In particular, the proportion of PBML cells that were CD11R1(+) cells, at end of test, was strongly correlated with daily gain (r(g) = -0.72; P < 0.01). There were also weaker but significant negative phenotypic correlations between PBML subsets measured at end of test and performance, for gamma delta(+) T cells, CD8 alpha(+), CD11R1(+) cells, B cells or monocytes. Phenotypic correlations with daily gain were generally lower at the start of test than at the end of test. These results show that most of the major pig PBML subsets are heritable, and that systemic levels of several of these PBML subsets are genetically negatively correlated with performance. This approach provides a basis for using immune trait markers when selecting boars that can produce higher-performing progeny.