Freezable and Unfreezable Hydration Water: Distinct Contributions to Protein Dynamics Revealed by Neutron Scattering
Freezable and Unfreezable Hydration Water: Distinct Contributions to Protein Dynamics Revealed by Neutron Scattering
复制标题
DOI：
10.1021/acs.jpclett.0c03786
复制
发表时间：
2021-02-25
影响因子：
5.7
通讯作者：
Shibayama, Naoya
中科院分区：
文献类型：
--
作者：
Yamamoto, Naoki;Kofu, Maiko;Shibayama, Naoya
水合水在激活功能表达所需的蛋白质动力学方面起着至关重要的作用。然而，关于水合水如何与蛋白质动力学耦合的细节还不清楚。水化水的冰形成的温度滞后是理解哪种类型的水化水（不可冻结的水化水或可冻结的水化水）对蛋白质动力学的激活至关重要的关键现象。使用中子散射，我们观察到的温度滞后现象的衍射峰的冰的可冻结的水合水，而蛋白质动力学没有表现出任何温度滞后。这些结果表明，蛋白质动力学与可冻结水化动力学不耦合，不可冻结水化水是激活蛋白质动力学的必要条件。非冻结和冻结水化水之间的动力学解耦可能是水化水对蛋白质动力学的不同贡献的原因。
Hydration water plays a crucial role for activating the protein dynamics required for functional expression. Yet, the details are not understood about how hydration water couples with protein dynamics. A temperature hysteresis of the ice formation of hydration water is a key phenomenon to understand which type of hydration water, unfreezable or freezable hydration water, is crucial for the activation of protein dynamics. Using neutron scattering, we observed a temperature-hysteresis phenomenon in the diffraction peaks of the ice of freezable hydration water, whereas protein dynamics did not show any temperature hysteresis. These results show that the protein dynamics is not coupled with freezable hydration water dynamics, and unfreezable hydration water is essential for the activation of protein dynamics. Decoupling of the dynamics between unfreezable and freezable hydration water could be the cause of the distinct contributions of hydration water to protein dynamics.