Cardiac, skeletal muscle and serum irisin responses to with or without water exercise in young and old male rats: Cardiac muscle produces more irisin than skeletal muscle
Cardiac, skeletal muscle and serum irisin responses to with or without water exercise in young and old male rats: Cardiac muscle produces more irisin than skeletal muscle
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DOI：
10.1016/j.peptides.2013.11.024
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发表时间：
2014-02-01
期刊：
影响因子：
3
通讯作者：
Dabak, Ozlem
中科院分区：
文献类型：
--
作者：
Aydin, Suna;Kuloglu, Tuncay;Dabak, Ozlem
鸢尾素将白色脂肪组织（WAT）转化为棕色脂肪组织（BAT），受能量消耗的调节。大鼠运动后鸢尾素浓度与人类运动后鸢尾素浓度之间的关系仍然存在争议。因此，我们：(1)在未运动的10周龄大鼠中测量心脏和骨骼肌、肝脏、肾脏、周围神经鞘和皮肤组织中鸢尾素的表达，以及血清中鸢尾素的水平；(2)测量心脏和骨骼肌组织上清中鸢尾素的水平，并对年轻和年老大鼠的运动做出反应，以确定哪些组织产生最多鸢尾素。实验采用幼龄大鼠（12个月）和老龄大鼠（24个月）进行或不进行10min运动（水上漂浮），10周龄健康的Sprague-Dawley大鼠不进行运动。未运动大鼠的骨骼肌切片中没有鸢尾素，只有肌膜被染色。心肌组织、外周髓鞘、肝脏、肾脏、皮肤真皮和皮下组织免疫反应性较强。未运动的幼龄和老年大鼠骨骼肌中未见鸢尾素，但运动后有少量鸢尾素。无论运动与否，年轻大鼠和老年大鼠心肌均表现出较强的免疫反应性，尤其是心包结缔组织。运动后血清鸢尾素增加，年轻大鼠高于年老大鼠。运动前后组织上清液（心肌和骨骼肌）中的鸢尾素含量均较高。高上清鸢尾素可能来自骨骼肌周围的结缔组织，尤其是位于其中的神经鞘。骨骼肌可能不是鸢尾素的主要来源。爱思唯尔公司版权所有版权所有。
Irisin converts white adipose tissue (WAT) into brown adipose tissue (BAT), as regulated by energy expenditure. The relationship between irisin concentrations after exercise in rats compared humans after exercise remains controversial. We therefore: (1) measured irisin expression in cardiac and skeletal muscle, liver, kidney, peripheral nerve sheath and skin tissues, as also serum irisin level in 10 week-old rats without exercise, and (2) measured tissue supernatant irisin levels in cardiac and skeletal muscle, and in response to exercise in young and old rats to establishing which tissues produced most irisin. Young (12 months) and old rats (24 months) with or without 10min exercise (water floating) and healthy 10 week-old Sprague-Dawley rats without exercise were used. Irisin was absent from sections of skeletal muscle of unexercised rats, the only part being stained being the perimysium. In contrast, cardiac muscle tissue, peripheral myelin sheath, liver, kidneys, and skin dermis and hypodermis were strongly immunoreactivity. No irisin was seen in skeletal muscle of unexercised young and old rats, but a slight amount was detected after exercise. Strong immunoreactivity occurred in cardiac muscle of young and old rats with or without exercise, notably in pericardial connective tissue. Serum irisin increased after exercise, being higher in younger than older rats. Irisin in tissue supernatants (cardiac and skeletal muscle) was high with or without exercise. High supernatant irisin could come from connective tissues around skeletal muscle, especially nerve sheaths located within it. Skeletal muscle is probably not a main irisin source. Copyright (C) 2013 Elsevier Inc. All rights reserved.