Identification and Phylogeny of the First T Cell Epitope Identified from a Human Gut Bacteroides Species.
Identification and Phylogeny of the First T Cell Epitope Identified from a Human Gut Bacteroides Species.
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DOI：
10.1371/journal.pone.0144382
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Peterson DA
中科院分区：
文献类型：
--
作者：
Perez-Muñoz ME;Joglekar P;Shen YJ;Chang KY;Peterson DA
宿主T细胞对肠道细菌表位的反应性被认为是疾病发病机制的一部分。然而，识别大量表位的T细胞的特异性还没有得到很好的描述。在C57BL/6J无菌小鼠体内定植人类肠道共生菌类杆菌后，我们分离出一株能在体外识别这些细菌的T细胞。利用这个T细胞，我们绘制了类杆菌门中第一个已知的非碳水化合物T细胞表位。T细胞还与另外两种类杆菌发生反应。我们用遗传学的方法鉴定了刺激T细胞的多肽。来自表位阳性和表位阴性细菌的基因组数据解释了T细胞对多种物种的交叉反应。这种表位的退化应该会塑造我们对健康和疾病状态下胃肠道复杂微生物群刺激的T细胞库的理解。
Host T cell reactivity toward gut bacterial epitopes has been recognized as part of disease pathogenesis. However, the specificity of T cells that recognize this vast number of epitopes has not yet been well described. After colonizing a C57BL/6J germ-free mouse with the human gut symbiotic bacteria Bacteroides thetaiotaomicron, we isolated a T cell that recognized these bacteria in vitro. Using this T cell, we mapped the first known non-carbohydrate T cell epitope within the phylum Bacteroidetes. The T cell also reacted to two other additional Bacteroides species. We identified the peptide that stimulated the T cell by using a genetic approach. Genomic data from the epitope-positive and epitope-negative bacteria explain the cross-reactivity of the T cell to multiple species. This epitope degeneracy should shape our understanding of the T cell repertoire stimulated by the complex microbiome residing in the gastrointestinal tract in both healthy and disease states.