Quantitative feature extraction reveals the status quo of protein fibrillation in the cell nucleus
Quantitative feature extraction reveals the status quo of protein fibrillation in the cell nucleus
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DOI：
10.1039/c0ib00146e
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发表时间：
2011-01-01
影响因子：
2.5
通讯作者：
von Mikecz, Anna
中科院分区：
文献类型：
--
作者：
Arnhold, Florian;von Mikecz, Anna
Stepwise fibrillation of otherwise soluble proteins to insoluble amyloid-like protein aggregates is a hallmark of neurodegenerative protein-misfolding diseases including Alzheimer's, polyglutamine diseases, and the prion encephalopathies. Investigation of protein aggregation mechanisms has considerably advanced in vitro due to recent technical innovation, whereas the development of analyses tools for intracellular protein fibrillation remains a major challenge. Here, we introduce a method that enables monitoring of the protein fibrillation status in the cell nucleus. We show that the amyloid indicator Congo red can be induced to bind to distinct nucleoplasmic microdomains that are describable by application of discrete mathematics on the image information. Since formation of Congo red-binding nuclear microdomains (CRBDs) correlates with increased amyloid formation and decreased solubility of endogenous proteins with homopolymeric polyglutamine (polyQ) stretches we introduce the idea that different protein fibrillation steps can be characterized intracellularly by graph theory-aided pattern recognition.
