Entomotoxic effects of fungal lectin from Rhizoctonia solani towards Spodoptera littoralis
Entomotoxic effects of fungal lectin from Rhizoctonia solani towards Spodoptera littoralis
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DOI：
10.1016/j.mycres.2009.10.002
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发表时间：
2010-01-01
期刊：
影响因子：
2.5
通讯作者：
Smagghe, G.
中科院分区：
文献类型：
--
作者：
Hamshou, M.;Van Damme, E. J. M.;Smagghe, G.
研究了立枯丝核菌凝集素（Rhizoctoniasolani lectin，RSA）对重要农业和园艺害虫--棉夜蛾（Spodoptera littoralis）生长发育和存活的影响。土壤病原菌R. Solani允许纯化大量的纯凝集素用于棉叶虫的饲养实验。饲养昆虫的饮食中含有不同浓度的RSA产生了强烈的影响，幼虫的体重增加。在第8天和第11天，在0.1%RSA的最低浓度下可见该效应。有趣的是，在1%RSA的情况下，在L 6结束时，幼虫重量显著降低89%，随后在处理的幼虫中出现82%的高死亡率。此外，化蛹和成虫形成的其他发育阶段也受到影响。此外，数据表明，RSA与Bt毒素的组合产生协同效应。0.03%RSA +0.005%Bt毒素处理，生长速率降低，死亡率升高.这些结果表明，RSA是一个有趣的工具，可用于生物工程抗虫在重要的农艺作物。(C)2009年英国真菌学会。由爱思唯尔有限公司出版。保留所有权利。
The effects of the Rhizoctonia solani lectin (RSA) on the growth, development and survival of an economically important caterpillar in agriculture and horticulture, the cotton leafworm, Spodoptera littoralis were studied. The high lectin concentration present in the sclerotes of the soil pathogen R. solani allowed the purification of large amounts of the pure lectin for feeding experiments with cotton leafworm. Rearing of insects on a diet containing different concentrations of RSA exerted a strong effect on the larval weight gain. This effect was visible at the lowest concentration of 0.1 % RSA at day 8 and day 11. Interestingly with 1 % RSA, there was a dramatic reduction in larval weight of 89 % at the end of L6 which was followed by a high mortality rate of 82 % in the treated larvae. Furthermore, the other developmental stages of pupation and adult formation were also affected. In addition, the data demonstrated that the combination of RSA with Bt toxin yielded synergistic effects. For instance, 0.03 % RSA + 0.005 % Bt toxin caused reduced growth rate and higher mortalities. These findings suggest that RSA is an interesting tool that can be used for bioengineering insect resistance in important agronomical crops. (C) 2009 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.