R(2)OBBIE-3D, a Fast Robotic High-Resolution System for Quantitative Phenotyping of Surface Geometry and Colour-Texture.

R(2)OBBIE-3D, a Fast Robotic High-Resolution System for Quantitative Phenotyping of Surface Geometry and Colour-Texture.
复制标题

DOI:
10.1371/journal.pone.0126740
复制
发表时间:
2015
期刊:
影响因子:
3.7
通讯作者:
Milinkovitch MC
Milinkovitch MC
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Martins AF;Bessant M;Manukyan L;Milinkovitch MC

文献摘要

参考文献

被引文献

相似文献

虽然最近的成像技术提供了从分子到细胞尺度的生物过程的见解,但更大规模的表型仍然很难进行定量分析。例如,对产生皮肤形态复杂性和多样性的生物物理机制的研究将极大地受益于 3D 几何和颜色纹理重建。在这里,我们报告 R2OBBIE-3D,这是一个集成系统,结合了机械臂、高分辨率数码彩色相机、高强度发光二极管照明篮和最先进的 3D 重建方法。我们证明,R2OBBIE 可以生成 1 至 100 cm 之间生物物体的精确 3D 模型,使多视图光度立体扫描在实际处理时间内成为可能,并且无需使用放大镜即可捕获优于 15 μm 的颜色纹理和几何分辨率。除了在生物医学等领域提供多种新应用之外,R2OBBIE 还具有极大改善表型定量分析的潜力。
While recent imaging techniques provide insights into biological processes from the molecular to the cellular scale, phenotypes at larger scales remain poorly amenable to quantitative analyses. For example, investigations of the biophysical mechanisms generating skin morphological complexity and diversity would greatly benefit from 3D geometry and colour-texture reconstructions. Here, we report on R2OBBIE-3D, an integrated system that combines a robotic arm, a high-resolution digital colour camera, an illumination basket of high-intensity light-emitting diodes and state-of-the-art 3D-reconstruction approaches. We demonstrate that R2OBBIE generates accurate 3D models of biological objects between 1 and 100 cm, makes multiview photometric stereo scanning possible in practical processing times, and enables the capture of colour-texture and geometric resolutions better than 15 μm without the use of magnifying lenses. R2OBBIE has the potential to greatly improve quantitative analyses of phenotypes in addition to providing multiple new applications in, e.g., biomedical science.
DOI: 10.1073/pnas.1213301109
发表时间: 2012-10-09
影响因子: 11.1
作者:
Harris, Andrew R.;Peter, Loic;Charras, Guillaume T.
通讯作者: Charras, Guillaume T.
DOI: 10.1016/j.patcog.2003.10.002
发表时间: 2004-04-01
影响因子: 8
作者:
Salvi, J;Pagès, J;Batlle, J
通讯作者: Batlle, J
DOI: 10.3390/s100807192
发表时间: 2010-08-01
期刊: SENSORS
影响因子: 3.9
作者:
Guidi, Gabriele;Russo, Michele;Bordegoni, Monica
通讯作者: Bordegoni, Monica
DOI: 10.1371/journal.pgen.0020061
发表时间: 2006-04
期刊: PLoS genetics
影响因子: 4.5
作者:
Johnson JT;Hansen MS;Wu I;Healy LJ;Johnson CR;Jones GM;Capecchi MR;Keller C
通讯作者: Keller C
DOI: 10.1038/nmeth.1586
发表时间: 2011-05
期刊: NATURE METHODS
影响因子: 48
作者:
Planchon, Thomas A.;Gao, Liang;Milkie, Daniel E.;Davidson, Michael W.;Galbraith, James A.;Galbraith, Catherine G.;Betzig, Eric
通讯作者: Betzig, Eric