Identification of novel cadherins expressed in human melanoma cells
Identification of novel cadherins expressed in human melanoma cells
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DOI：
10.1111/1523-1747.ep12292703
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发表时间：
1997-06-01
影响因子：
6.5
通讯作者：
Imamura, S
中科院分区：
文献类型：
--
作者：
Matsuyoshi, N;Tanaka, T;Imamura, S
钙粘蛋白分子对于组织形态发生至关重要，也与癌症侵袭和转移有关。尽管正常的黑素细胞表达E-和P-钙粘蛋白，但e-和p-钙粘着蛋白在黑色素瘤细胞中的活性和表达仍然未知，我们测量了人正常表皮黑色素细胞和黑色素瘤细胞系MEWO和A375，A375，A375，A375，A375，A375，A375，A375（黑色素瘤细胞表现出比黑色素细胞在黑色素瘤细胞中e-和p-钙粘着蛋白表达较低的黑色素细胞表现更强，但这种结果表明，黑色素瘤细胞使用脱氧化剂来扩增多重cadherin usphherin upprimers，以扩增其他类型的均质粘附分子，我们与黑色素瘤细胞的mRNA逆转录产生的CDNA的第一线进行了聚合酶链反应（PCR），我们分离了两个已知的钙粘蛋白片段N-核蛋白N-钙粘着蛋白和PC42，以及六个新型的钙粘蛋白片段，Cadherins ME1 ME1 ME1 ME1 -me6，使用特定的钙粘蛋白引物（包括E-，P-和N-核蛋白），PC42和Cadherins ME1-ME6的逆转录酶-PCR-Me6显示，黑色素瘤细胞比黑色素形成蛋白表达更多的钙粘蛋白分子。这种钙粘蛋白可能在黑色素瘤细胞 - 细胞粘附中起重要作用。
Cadherin molecules are essential for tissue morphogenesis and are also related to cancer invasion and metastasis. Although normal melanocytes express E- and P-cadherin, the activity and expression of E- and P-cadherin in melanoma cells are still unknown, We measured the homophilic adhesion activity of human normal epidermal melanocytes and the melanoma cell lines MeWo and A375, The melanoma cells showed stronger homophilic adhesion activity than did the melanocytes, despite the lower expression of E- and P-cadherin in the melanoma cells, This result suggested that melanoma cells expressed other types of homophilic adhesion molecules, Using degenerate primers to amplify multiple cadherin subtypes, we performed a polymerase chain reaction (PCR) with the first strand of cDNAs generated by reverse transcription of the mRNAs of the melanoma cells, and we isolated two known cadherin fragments, N-cadherin and PC42, and six novel cadherin fragments, cadherins ME1-ME6, The reverse transcriptase-PCR using specific primers of cadherins including E-, P-, and N-cadherins, PC42, and cadherins ME1-ME6 revealed that the melanoma cells expressed more kinds of cadherin molecules than did the melanocytes. Such cadherins may play an important role in melanoma cell-cell adhesion.