A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering
A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering
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DOI：
10.1016/s0142-9612(02)00635-x
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发表时间：
2003-05-01
期刊：
影响因子：
14
通讯作者：
Vacanti, JP
中科院分区：
文献类型：
--
作者：
Yoshimoto, H;Shin, YM;Vacanti, JP
采用静电纺丝法制备了聚己内酯（PCL）多孔非织造支架。在该方法中，通过使流体射流经受高电场来形成直径低至纳米范围的聚合物纤维或纳米纤维。从新生大鼠骨髓中分离培养间充质干细胞（MSCs），扩增后接种于油电纺PCL支架上。在动态培养条件下，将细胞-聚合物构建体与成骨补充剂一起培养长达4周。细胞-聚合物构建体保持了原始支架的大小和形状。进行扫描电镜、组织学和免疫组化检查。1周后，在细胞-聚合物构建体中观察到细胞的渗透和丰富的细胞外基质。SEM显示，在4周时，细胞-聚合物构建体的表面被细胞多层覆盖。此外，在4周时观察到矿化和I型胶原。这表明静电纺丝PCL是一种潜在的骨组织工程支架材料。(C)2003爱思唯尔科技有限公司版权所有。
Microporous, non-woven poly(epsilon-caprolactone) (PCL) scaffolds were made by electrostatic fiber spinning. In this process, polymer fibers with diameters down to the nanometer range, or nanofibers, are formed by subjecting a fluid jet to a high electric field. Mesenchymal stem cells (MSCs) derived from the bone marrow of neonatal rats were cultured, expanded and seeded oil electrospun PCL scaffolds. The cell-polymer constructs were cultured with osteogenic supplements under dynamic culture conditions for up to 4 weeks. The cell-polymer constructs maintained the size and shape of the original scaffolds. Scanning electron microscopy (SEM), histological and immunohistochemical examinations were performed. Penetration of cells and abundant extracellular matrix were observed in the cell-polymer constructs after 1 week. SEM showed that the surfaces of the cell-polymer constructs were covered with cell multilayers at 4 weeks. In addition, mineralization and type I collagen were observed at 4 weeks. This suggests that electrospun PCL is a potential candidate scaffold for bone tissue engineering. (C) 2003 Elsevier Science Ltd. All rights reserved.