ZINC ACETATE AS A PRECIPITANT OF UNSTABLE HEMOGLOBINS
ZINC ACETATE AS A PRECIPITANT OF UNSTABLE HEMOGLOBINS
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DOI：
10.1136/jcp.34.7.796
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发表时间：
1981-01-01
影响因子：
3.4
通讯作者：
LEHMANN, H
中科院分区：
文献类型：
--
作者：
CARRELL, RW;LEHMANN, H
在pH值为7.4时，将4摩尔的醋酸锌加入到正常人的Hb中，可得到透明的溶液，而加入不稳定的Hb则会产生沉淀。在一定范围内，高铁血红蛋白和胎儿Hb均无假阳性结果。在锌过量的情况下，正常的Hb也会析出，但这个过程是可逆的，可以用于快速浓缩正常Hb的稀溶液。
Whereas the addition of 4 mol of zinc acetate to one of normal human Hb at pH 7.4 results in a clear solution, addition to unstable Hb results in precipitate formation. Within certain limits no false-positive results are obtained with methemoglobin, and with fetal Hb. With excess Zn, normal Hb also precipitates, but this process is reversible and can be used for a rapid concentration of dilute solutions of normal Hb.