The antimicrobial activity and biocompatibility of a controlled gentamicin-releasing single-layer sol-gel coating on hydroxyapatite-coated titanium.
The antimicrobial activity and biocompatibility of a controlled gentamicin-releasing single-layer sol-gel coating on hydroxyapatite-coated titanium.
复制标题
羟基磷灰石包覆钛上控制庆大霉素释放的单层溶胶-凝胶涂层的抗菌活性和生物相容性。
DOI：
10.1302/0301-620x.103b3.bjj-2020-0347.r1
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Akid R
中科院分区：
文献类型：
--
作者：
Nichol T;Callaghan J;Townsend R;Stockley I;Hatton PV;Le Maitre C;Smith TJ;Akid R
本研究的目的是开发一种单层混合有机-无机溶胶-凝胶涂层，该涂层能够控制非骨水泥型羟基磷灰石（HA）涂层钛骨科假体的抗生素释放。制备含有浓度为1.25%重量/体积（wt/vol）的庆大霉素的涂层，类似于市售的骨水泥中发现的涂层，并在实验室中测试：抗生素释放动力学;对抗生素和生物膜细菌培养物的活性;与培养的哺乳动物细胞的生物相容性;以及与材料的物理结合（所有测试中n = 3）。然后在小动物愈合模型（测试四种材料;每种材料n = 6）中体内测试溶胶-凝胶涂层和对照，并将其施加到商业纯HA涂覆的钛棒的表面。涂层在1小时内释放庆大霉素，其对敏感葡萄球菌菌株的最小抑菌浓度（MIC）> 10倍，从而可能有效预防关节成形术，并显示48小时后涂层内抗生素洗脱> 99%。一组临床相关葡萄球菌的致病菌和已建立的细菌生物膜均被完全根除。间充质干细胞粘附在涂层表面并向成骨细胞分化，沉积钙并表达骨标记蛋白骨桥蛋白。在体内小动物骨愈合模型中，抗生素溶胶-凝胶涂层钛（Ti）/HA棒导致与传统HA涂层表面等同的骨整合。在这项研究中，我们报告了一种新的溶胶-凝胶技术，可以从生物陶瓷涂层的非骨水泥关节置换材料中释放庆大霉素。在体外，局部庆大霉素水平超过骨水泥所能达到的水平。在体内，动物模型中的骨愈合未受损。因此，这代表了一种生物材料改良，可能有可能保护风险患者免受植入物相关深部感染。引用这篇文章：骨关节J 2021;103-B（3）：522-529。
The aim of this study was to develop a single-layer hybrid organic-inorganic sol-gel coating that is capable of a controlled antibiotic release for cementless hydroxyapatite (HA)-coated titanium orthopaedic prostheses. Coatings containing gentamicin at a concentration of 1.25% weight/volume (wt/vol), similar to that found in commercially available antibiotic-loaded bone cement, were prepared and tested in the laboratory for: kinetics of antibiotic release; activity against planktonic and biofilm bacterial cultures; biocompatibility with cultured mammalian cells; and physical bonding to the material (n = 3 in all tests). The sol-gel coatings and controls were then tested in vivo in a small animal healing model (four materials tested; n = 6 per material), and applied to the surface of commercially pure HA-coated titanium rods. The coating released gentamicin at > 10 × minimum inhibitory concentration (MIC) for sensitive staphylococcal strains within one hour thereby potentially giving effective prophylaxis for arthroplasty surgery, and showed > 99% elution of the antibiotic within the coating after 48 hours. There was total eradication of both planktonic bacteria and established bacterial biofilms of a panel of clinically relevant staphylococci. Mesenchymal stem cells adhered to the coated surfaces and differentiated towards osteoblasts, depositing calcium and expressing the bone marker protein, osteopontin. In the in vivo small animal bone healing model, the antibiotic sol-gel coated titanium (Ti)/HA rod led to osseointegration equivalent to that of the conventional HA-coated surface. In this study we report a new sol-gel technology that can release gentamicin from a bioceramic-coated cementless arthroplasty material. In vitro, local gentamicin levels are in excess of what can be achieved by antibiotic-loaded bone cement. In vivo, bone healing in an animal model is not impaired. This, thus, represents a biomaterial modification that may have the potential to protect at-risk patients from implant-related deep infection. Cite this article: Bone Joint J 2021;103-B(3):522–529.