Cloning of primary human tumors in capillary tube versus Petri dish: a head-to-head comparison.
Cloning of primary human tumors in capillary tube versus Petri dish: a head-to-head comparison.
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毛细管与培养皿中原发性人类肿瘤的克隆：头对头比较。
DOI：
10.1159/000163604
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
MurphyJr,MJ
中科院分区：
文献类型：
--
作者：
Peng,XG;MurphyJr,MJ
将毛细管测定系统中克隆的原发性人类肿瘤的接种效率（PE）与传统两层琼脂培养皿测定系统中获得的接种效率进行比较。在两个检测系统中同时测试了总共 143 个连续接收的 24 种不同病理的原发性人类肿瘤。毛细管测定中的成功克隆生长率为 82.7%，而培养皿中的成功克隆生长率为 64.7% (&p<0.001)。毛细管测定中的中位 PE 为 0.017%，比培养皿系统的 0.004% PE 增加了 4.25 倍。这些数据证实了之前的结果，即卵巢、乳腺癌和肺来源的癌细胞在毛细管中克隆时具有较高的 PE。相比之下，我们通过培养皿方法证实胃癌细胞是唯一表现出较高 PE 的肿瘤类型。此外，这项研究首次表明，淋巴瘤和肾细胞癌在体外存活时，在两种方法中克隆得同样好。然而，毛细管克隆方法的淋巴瘤细胞克隆成功率为66%，而培养皿克隆的成功率仅为33%。因此，对于某些类型的癌症（即淋巴瘤），毛细管克隆可能是有利的，因为它提高了获得可评估结果的可能性。在其他情况下，毛细管克隆的优点可能只是减少了测定所需的样本和试剂的数量。
Plating efficiencies (PEs) of primary human tumors cloned in a capillary assay system were compared to those derived from the conventional two-layer agar Petri dish assay system. A total of 143 consecutively received primary human tumors of 24 different pathologies were simultaneously tested in both assay systems. The successful clonal growth rate in the capillary assay was 82.7%, while in the Petri dish it was 64.7% (&p<0.001). The median PE was 0.017% in the capillary assay demonstrating a 4.25-fold increase over the 0.004% PE of the Petri dish system. The data confirmed previous results showing that cancer cells of ovarian, breast, and lung origins clone with higher PE in capillary tubes. In contrast, we confirmed that stomach carcinoma cells were the only tumor type that showed a higher PE with the Petri dish method. In addition, this study shows for the first time that lymphomas and renal cell carcinoma, when they survive in vitro, clone equally well in both methods. However, the capillary cloning method resulted in a 66% success rate for lymphoma cell cloning, but Petri dish cloning resulted in only a 33% success rate. Thus, for some types of cancers (i.e., lymphoma), capillary cloning may be advantageous because it improves the probability of obtaining evaluable results. In other cases, the advantage of capillary cloning may be only the decreased amount of specimen and reagents needed for the assay.