Effects of exhaustive endurance exercise and its one-week daily repetition on neutrophil count and functional status in untrained men
Effects of exhaustive endurance exercise and its one-week daily repetition on neutrophil count and functional status in untrained men
复制标题
DOI：
10.1055/s-2007-972833
复制
发表时间：
1996-04-01
影响因子：
2.5
通讯作者：
Sugawara, K
中科院分区：
文献类型：
--
作者：
Suzuki, K;Naganuma, S;Sugawara, K
虽然耐力运动已知会诱发明显的嗜中性粒细胞，但细胞功能是否改变以及对训练的反应是否发生适应性仍有待充分理解。为了解决这两个问题，我们对10名健康的未经训练的男性（20-24岁，VO（2）max 39.1 +/- 4.2 ml/kg/min）进行了纵向研究。运动方案为每天连续进行7次相同负荷的运动，持续1.5 h，强度为VO（2）max的70%。在第1、4和7天运动前、运动后即刻和运动后1 h采集外周血样本，用于测定总白细胞计数和白细胞分类计数、中性粒细胞趋化性和化学发光。急性耐力运动引起外周血嗜中性粒细胞明显增多，带状中性粒细胞绝对数量和相对比例均显著增加（P < 0.01），表明骨髓中性粒细胞部分募集。虽然运动后趋化性保持不变，但中性粒细胞的活性氧产生（用调理剂酵母聚糖刺激后鲁米诺依赖性化学发光测定）不仅在运动后显著增强，（p < 0.01），但也与带状中性粒细胞的比例增加有关（r = 0.727，p < 0.05），提示耐力运动后骨髓动员的中性粒细胞可能具有更高的反应性。另一方面，运动引起的变化的幅度逐渐减少，每天重复暴露于耐力运动，但没有任何趋势是显着的，除了静息分叶中性粒细胞计数下降（p < 0.05），至少在1周的重复运动期。
Whereas endurance exercise is known to induce marked neutrophilia, it remains to be fully understood as to whether the cell functions are altered as well as whether the adaptability of the responses to training occurs. To address both of these issues, we undertook the present longitudinal investigation in ten healthy untrained men (20-24 years, VO(2)max 39.1 +/- 4.2 ml/kg/min). The exercise protocol was 7 consecutive sessions of the same workload performed each day for 1.5 h at an intensity of 70 % of VO(2)max. Peripheral blood samples were obtained before, immediately after, and 1 h after exercise on Days 1, 4, and 7, and served for determination of total and differential leukocyte counts, chemotaxis and chemiluminescence of neutrophils. Acute endurance exercise caused marked peripheral neutrophilia with significant increase in both absolute number and relative proportion of band neutrophils (p < 0.01, respectively), indicating partial recruitment of bone marrow neutrophils. While chemotaxis remained unaltered following exercise, reactive oxygen species generation of neutrophils, measured by luminol-dependent chemiluminescence upon stimulation with opsonized zymosan, was not only significantly enhanced following exercise (p < 0.01), but also associated with the proportional increase in band neutrophils (r = 0.727, p < 0.05), suggesting that neutrophils mobilized from the bone marrow following endurance exercise may possess higher responsiveness. On the other hand, the magnitude of the exercise-induced changes was reduced gradually by daily repeated exposure to endurance exercise, but none of the trends were significant except the decline in resting segmented neutrophil counts (p < 0.05) at least during a 1-wk period of repeated exercise sessions.