Antibacterial AgNPs-PAAm-CS-PVP nanocomposite hydrogel coating for urinary catheters
Antibacterial AgNPs-PAAm-CS-PVP nanocomposite hydrogel coating for urinary catheters
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DOI：
10.1016/j.eurpolymj.2023.112260
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发表时间：
2023-09
影响因子：
6
通讯作者：
Yongwei Cai;Han Yang;Jianxiang Li;R. Gu;Yuhang Dong;Qi Zhao;Yao-Cyong Chen;Yuan Li;Ruru Wang
中科院分区：
文献类型：
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作者：
Yongwei Cai;Han Yang;Jianxiang Li;R. Gu;Yuhang Dong;Qi Zhao;Yao-Cyong Chen;Yuan Li;Ruru Wang
导管相关性尿路感染 (CAUTI) 占所有医院获得性感染的 40%，美国和欧洲每年发生超过 100 万例 CAUTI，且发病率和死亡率不断增加。尽管抗菌涂层是解决这一问题的有效途径，但迄今为止只取得了有限的进展。本文通过简单的浸渍方法在乳胶导尿管上制备了一种新型抗菌纳米银聚(丙烯酰胺)-壳聚糖-聚乙烯吡咯烷酮(AgNPs-PAAm-CS-PVP)复合水凝胶涂层。通过 TEM 分析检查了 AgNP 的平均粒径、尺寸分布以及多分散指数 (PDI) 值。利用SEM、EDS、AFM、FTIR、XPS和视频光学接触角仪分别对涂层的表面形貌和粗糙度、元素含量、官能团、静态接触角和表面能进行表征。通过ICP-MS测试从水凝胶中释放的总Ag量。对革兰氏阴性大肠杆菌有抗菌和抗粘连作用。通过抑菌圈测试和荧光显微镜对涂覆导尿管的大肠杆菌进行评估。还提出了 AgNPs 和 CS 的协同抗菌机制。结果表明，水凝胶中合成的 AgNP 呈球形，平均直径为 25.9 nm。 AgNPs-PAAm-CS-PVP 水凝胶能够更持久地释放 AgNPs 和 Ag+ 的抗菌剂，这解释了为什么涂层导尿管具有长期抗菌活性。 Ag 释放动力学的数学模型支持 AgNPs-PAAm-CS-PVP 水凝胶的案例 I 或 Fickian 扩散机制。水凝胶涂层导尿管变得更光滑、更亲水，这进一步解释了为什么涂层导尿管减少了细菌粘附。更重要的是，细菌活性和细菌粘附测定表明，AgNPs-PAAm-CS-PVP水凝胶涂层导尿管的外表面和内表面均对大肠杆菌具有较强的抗菌性能。大肠杆菌。此外，水凝胶涂层导尿管表现出高血液相容性和细胞相容性，具有广阔的生物医学应用前景。 AgNPs-PAAm-CS-PVP 水凝胶涂层导尿管在减少 CAUTI 方面具有巨大潜力。
Catheter-associated urinary tract infections (CAUTI) account for 40% of all hospital-acquired infections and more than 1 million CAUTIs occur annually in the United States and Europe with increased morbidity and mortality. Although anti-bacterial coating is an effective way to solve this problem, so far only limitted progress has been made. In this paper we prepared a novel antibacterial silver nanoparticles poly(acrylamide)-chitosan-polyvinylpyrrolidone (AgNPs-PAAm-CS-PVP) composite hydrogel coating on latex urinary catheters by a simple dipping method. The average particle size, the size distribution as well as the polydispersity index (PDI) value of the AgNPs were examined by using TEM analysis. The surface morphology and roughness, the element contents, the functional groups, the static contact angles, and the surface energy of the coatings were characterized by using SEM, EDS, AFM, FTIR, XPS, and a video optical contact angle instrument, respectively. The amount of the total Ag released from the hydrogel was tested by ICP-MS. The antibacterial and antiadhesion efficacies against Gram-negativeE. coliof the coated urinary catheters were evaluated with an inhibition zone test and fluorescence microscopy. A synergistic antibacterial mechanism of AgNPs and CS was also presented. The results showed that the synthesized AgNPs in the hydrogel had a spherical shape with an average diameter of 25.9 nm. The AgNPs-PAAm-CS-PVP hydrogel had a more durable release of the antibacterial agents of AgNPs and Ag+, which explains why the coated urinary catheters had long-term antibacterial activity. The mathematical modelling of Ag release kinetics supported Case I or Fickian diffusion mechanism for the AgNPs-PAAm-CS-PVP hydrogel. The hydrogel-coated urinary catheters became smoother and more hydrophilic which further explains why the coated urinary catheter decreased bacterial adhesion. More importantly, bacterial activity and bacterial adhesion assays showed that both the outer and inner surfaces of the AgNPs-PAAm-CS-PVP hydrogel-coated urinary catheters had strong antibacterial property againstE. coli. Furthermore, the hydrogel-coated urinary catheters showed high hemocompatibility and cytocompatibility for promising biomedical applications. The AgNPs-PAAm-CS-PVP hydrogel-coated urinary catheters have great potential for decreasing CAUTIs.