Production of CFTR-ΔF508 Rabbits.
Production of CFTR-ΔF508 Rabbits.
复制标题
CFTR-ΔF508兔子的产生。
DOI：
10.3389/fgene.2020.627666
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发表时间：
2020
影响因子：
3.7
通讯作者：
Xu J
中科院分区：
文献类型：
--
作者：
Yang D;Liang X;Pallas B;Hoenerhoff M;Ren Z;Han R;Zhang J;Chen YE;Jin JP;Sun F;Xu J
囊性纤维化（CF）是一种致死性常染色体隐性遗传疾病，由编码囊性纤维化跨膜传导调节因子（CFTR）的基因突变引起。最常见的突变是508位苯丙氨酸残基的缺失（ΔF508）。在这里，我们报告了通过CRISPR/Cas9介导的基因编辑产生CFTR-ΔF508兔。在显微注射和胚胎移植后，77个试剂盒出生，其中5个携带ΔF508突变。为了确认生殖系传播，将一只雄性ΔF508建立者与两只野生型雌性交配，产生16只F1代试剂盒，其中6只为杂合ΔF508/WT动物。我们的工作将CFTR-ΔF508兔添加到CF动物模型工具箱中，用于生物医学研究。
Cystic Fibrosis (CF) is a lethal autosomal recessive disease caused by mutations in the gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR). The most common mutation is the deletion of phenylalanine residue at position 508 (ΔF508). Here we report the production of CFTR-ΔF508 rabbits by CRISPR/Cas9-mediated gene editing. After microinjection and embryo transfer, 77 kits were born, of which five carried the ΔF508 mutation. To confirm the germline transmission, one male ΔF508 founder was bred with two wild-type females and produced 16 F1 generation kits, of which six are heterozygous ΔF508/WT animals. Our work adds CFTR-ΔF508 rabbits to the toolbox of CF animal models for biomedical research.