HERPES-SIMPLEX VIRUS TYPE-1 AND TYPE-2 COMPLETELY HELP ADENOVIRUS-ASSOCIATED VIRUS-REPLICATION
HERPES-SIMPLEX VIRUS TYPE-1 AND TYPE-2 COMPLETELY HELP ADENOVIRUS-ASSOCIATED VIRUS-REPLICATION
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DOI：
10.1128/jvi.40.1.241-247.1981
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发表时间：
1981-01-01
影响因子：
5.4
通讯作者：
ROSE, JA
中科院分区：
文献类型：
--
作者：
BULLER, RML;JANIK, JE;ROSE, JA
除了能够完全帮助腺病毒相关病毒（AAV）增殖的腺病毒外，已知只有疱疹病毒提供任何AAV辅助活性，但这种活性被认为是部分的（即，诱导AAV， DNA， RNA和蛋白质合成，但不组装感染性颗粒）。单纯疱疹病毒1型（HSV-1）和2型（HSV-2）是完全的AAV辅助病毒，AAV2型（AAV2）的传染性产率可以接近与辅助腺病毒共感染时获得的产率。在2株HSV-1（11124和17MP）和1株HSV-2 （HG52）的KB[人喉癌]细胞中证实了AAV辅助活性。每种疱疹病毒都以相当的效率支持AAV2增殖。AAV2在HSV-1合并感染的HeLa细胞（人宫颈癌）中增殖效率相似，而在HEp-2（人咽癌）和原代人胚胎肾细胞中增殖效率较低。HSV-1也支持AAV1在HeLa细胞中的增殖，但在相应的感染倍数下，AAV1的增殖效率低于AAV2。比较5型腺病毒和1型单纯疱疹病毒合并感染后AAV2 DNA、RNA和蛋白质合成的时间过程发现，与1型单纯疱疹病毒合并感染时，AAV2开始合成和达到最大合成率的时间都较早。这些发现将AAV大分子合成与辅助病毒周期中的一个事件联系起来。除了这种时间上的关联外，AAV大分子合成中帮助者相关的差异并不明显。
In addition to adenoviruses, which are capable of completely helping adenovirus-associated virus (AAV) multiplication, only herpesviruses are known to provide any AAV helper activity, but this activity is thought to be partial (i.e., AAV, DNA, RNA and protein syntheses are induced but infectious particles are not assembled). Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) are complete AAV helpers and AAV type 2 (AAV2) infectivity yields can approach those obtained when coinfections are carried out with a helper adenovirus. AAV helper activity was demonstrated in KB [human laryngeal carcinoma] cells with 2 HSV-1 strains (11124 and 17MP) and an HSV-2 strain (HG52). Each herpesvirus supported AAV2 multiplication with comparable efficiency. AAV2 multiplication was similarly efficient in HSV-1 coinfections of HeLa cells [human cervical carcinoma], whereas lower yields were obtained in HEp-2 [human pharyngeal carcinoma] and primary human embryonic kidney cells. HSV-1 also supported AAV1 multiplication in HeLa cells but, at corresponding multiplicities of infection, AAV1 grew less efficiently than AAV2. Comparisons of the time courses of AAV2 DNA, RNA and protein syntheses after coinfection with either adenovirus type 5 or HSV-1 revealed that, in each case, the onset of synthesis and attainment of maximal synthesis rate occurred earlier in coinfections with HSV-1. These findings link AAV macromolecular synthesis to an event(s) in the helper virus cycle. Aside from this temporal association, helper-related differences in AAV macromolecular synthesis were not apparent.