Jasmonate signaling is activated in the very early stages of iron deficiency responses in rice roots.
Jasmonate signaling is activated in the very early stages of iron deficiency responses in rice roots.
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DOI：
10.1007/s11103-016-0486-3
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发表时间：
2016-07
影响因子：
5.1
通讯作者：
Nishizawa NK
中科院分区：
文献类型：
--
作者：
Kobayashi T;Itai RN;Senoura T;Oikawa T;Ishimaru Y;Ueda M;Nakanishi H;Nishizawa NK
在低铁条件下，植物在转录水平上诱导参与铁吸收和转运的各种基因的表达。这种缺铁反应受各种植物激素的影响，但茉莉酸在这种反应中的作用还不清楚。我们调查了茉莉酸在水稻缺铁反应的参与。在缺铁处理的早期，水稻根系中茉莉酸诱导基因的表达率较高。许多茉莉酸诱导的基因也负调控的泛素连接酶OsHRZ 1和OsHRZ 2和正调控的转录因子IDEF 1。10出35个基因参与茉莉酸的生物合成和信号转导迅速诱导在3小时的缺铁处理，这种诱导之前，已知的铁缺乏诱导基因参与铁的吸收和转运。12个基因参与茉莉酸的生物合成和信号也上调HRZ敲低根。内源浓度的茉莉酸和茉莉酰异亮氨酸往往迅速增加根中响应缺铁处理，而这些浓度较高的HRZ敲低根铁充足的条件下。茉莉酸缺乏突变体的分析表明，茉莉酸抑制许多铁缺乏诱导基因的表达，参与铁的吸收和转运铁充足，但这种抑制在铁缺乏的早期阶段部分取消。这些结果表明，茉莉酸信号在铁缺乏的早期阶段被激活，这部分是由IDEF 1和OsHRZs调节。本文的在线版本（doi：10.1007/s11103-016-0486-3）包含补充材料，可供授权用户使用。
Under low iron availability, plants induce the expression of various genes involved in iron uptake and translocation at the transcriptional level. This iron deficiency response is affected by various plant hormones, but the roles of jasmonates in this response are not well-known. We investigated the involvement of jasmonates in rice iron deficiency responses. High rates of jasmonate-inducible genes were induced during the very early stages of iron deficiency treatment in rice roots. Many jasmonate-inducible genes were also negatively regulated by the ubiquitin ligases OsHRZ1 and OsHRZ2 and positively regulated by the transcription factor IDEF1. Ten out of 35 genes involved in jasmonate biosynthesis and signaling were rapidly induced at 3 h of iron deficiency treatment, and this induction preceded that of known iron deficiency-inducible genes involved in iron uptake and translocation. Twelve genes involved in jasmonate biosynthesis and signaling were also upregulated in HRZ-knockdown roots. Endogenous concentrations of jasmonic acid and jasmonoyl isoleucine tended to be rapidly increased in roots in response to iron deficiency treatment, whereas these concentrations were higher in HRZ-knockdown roots under iron-sufficient conditions. Analysis of the jasmonate-deficient cpm2 mutant revealed that jasmonates repress the expression of many iron deficiency-inducible genes involved in iron uptake and translocation under iron sufficiency, but this repression is partly canceled under an early stage of iron deficiency. These results indicate that jasmonate signaling is activated during the very early stages of iron deficiency, which is partly regulated by IDEF1 and OsHRZs. The online version of this article (doi:10.1007/s11103-016-0486-3) contains supplementary material, which is available to authorized users.