Rapid detection of highly pathogenic avian influenza H5N1 virus by TaqMan reverse transcriptase-polymerasc chain reaction
Rapid detection of highly pathogenic avian influenza H5N1 virus by TaqMan reverse transcriptase-polymerasc chain reaction
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DOI：
10.1637/7587-040206r.1
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发表时间：
2007-01-01
期刊：
影响因子：
1.4
通讯作者：
Lowther, S.
中科院分区：
文献类型：
--
作者：
Heine, H. G.;Trinidad, L.;Lowther, S.
自2003年底以来，高致病性禽流感（AI）H5 N1病毒一直从亚洲传播。早期发现和分类对于控制疾病至关重要，因为这些病毒对鸟类是致命的，并导致人类死亡。在这里，我们描述了TaqMan逆转录酶-聚合酶链反应检测快速检测所有禽流感病毒（A型流感），并确定H5 N1的欧亚血统。该检测试剂盒在10（5）-10（6）线性范围内具有灵敏度和定量性，可检出所有供试AI病毒，并能够区分H5和H7亚型。这些测试可在数小时内确定禽流感病毒为H5，这对于在爆发时迅速实施控制措施至关重要。
Highly pathogenic avian influenza (AI) H5N1 viruses have been spreading from Asia since late 2003. Early detection and classification are paramount for control of the disease because these viruses are lethal to birds and have caused fatalities in humans. Here, we described TaqMan reverse transcriptase-polymerase chain reaction assays for rapid detection of all AI viruses (influenza type A) and for identification of H5N1 of the Eurasian lineage. The assays were sensitive and quantitative over a 10(5)-10(6) linear range, detected all of the tested AI viruses, and enabled differentiation between H5 and H7 subtypes. These tests allow definitive confirmation of an AI virus as H5 within hours, which is crucial for rapid implementation of control measures in the event of an outbreak.