Transcriptome Analysis of Early Responsive Genes in Rice during Magnaporthe oryzae Infection.
Transcriptome Analysis of Early Responsive Genes in Rice during Magnaporthe oryzae Infection.
复制标题
麦糖蛋白酶感染过程中水稻早期反应基因的转录组分析。
DOI：
10.5423/ppj.oa.06.2014.0055
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发表时间：
2014-12
期刊：
影响因子：
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通讯作者：
Kim ST
中科院分区：
文献类型：
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作者：
Wang Y;Kwon SJ;Wu J;Choi J;Lee YH;Agrawal GK;Tamogami S;Rakwal R;Park SR;Kim BG;Jung KH;Kang KY;Kim SG;Kim ST
由稻瘟病菌引起的稻瘟病是栽培稻上最严重的病害之一。在世界上大多数水稻种植地区。为了研究水稻的早期反应基因，我们利用300K基因芯片的转录组分析方法，对感染亲和型和不亲和型稻曲霉菌株的水稻叶片进行了分析。在基因芯片实验之前，通过RT-PCR检测水稻防御相关标记基因(几丁质酶2、Barwin、PBZ1和PR-10)和植物保卫素(樱花素和吗内酯A)的差异表达来验证总RNA。微阵列分析显示，与亲和互作相比，231个基因在不亲和互作中上调(>；2倍变化，p<；0.05)。高表达基因从功能上分为代谢过程和氧化还原两类。氧化应激反应在感染的早期和后期都被诱导。来自MapMan分析的生物胁迫概述表明，植物激素乙烯以及信号分子茉莉酸和水杨酸对防御基因的调控是重要的。WRKY和Myb转录因子也参与了信号转导过程。此外，受体样激酶更可能与防御反应相关，它们的表达模式通过RT-PCR得到了验证。我们的结果表明，候选基因，包括受体样蛋白激酶，可能在对米曲霉攻击的抗病中发挥关键作用。
Rice blast disease caused by Magnaporthe oryzae is one of the most serious diseases of cultivated rice (Oryza sativa L.) in most rice-growing regions of the world. In order to investigate early response genes in rice, we utilized the transcriptome analysis approach using a 300 K tilling microarray to rice leaves infected with compatible and incompatible M. oryzae strains. Prior to the microarray experiment, total RNA was validated by measuring the differential expression of rice defense-related marker genes (chitinase 2, barwin, PBZ1, and PR-10) by RT-PCR, and phytoalexins (sakuranetin and momilactone A) with HPLC. Microarray analysis revealed that 231 genes were up-regulated (>2 fold change, p < 0.05) in the incompatible interaction compared to the compatible one. Highly expressed genes were functionally characterized into metabolic processes and oxidation-reduction categories. The oxidative stress response was induced in both early and later infection stages. Biotic stress overview from MapMan analysis revealed that the phytohormone ethylene as well as signaling molecules jasmonic acid and salicylic acid is important for defense gene regulation. WRKY and Myb transcription factors were also involved in signal transduction processes. Additionally, receptor-like kinases were more likely associated with the defense response, and their expression patterns were validated by RT-PCR. Our results suggest that candidate genes, including receptor-like protein kinases, may play a key role in disease resistance against M. oryzae attack.