Protein Profiling of Arabidopsis Roots Treated With Humic Substances: Insights Into the Metabolic and Interactome Networks
Protein Profiling of Arabidopsis Roots Treated With Humic Substances: Insights Into the Metabolic and Interactome Networks
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DOI：
10.3389/fpls.2018.01812
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发表时间：
2018-12
影响因子：
5.6
通讯作者：
S. Roomi;A. Masi;G. Conselvan;S. Trevisan;S. Quaggiotti;Micaela Pivato;G. Arrigoni;T. Yasmin;P. Carletti
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文献类型：
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作者：
S. Roomi;A. Masi;G. Conselvan;S. Trevisan;S. Quaggiotti;Micaela Pivato;G. Arrigoni;T. Yasmin;P. Carletti
背景和目的：腐殖质（HS）影响土壤的化学和物理特性，并且还影响植物生理和养分吸收。本研究旨在阐明受 HS 活性影响的植物代谢途径和生理过程。方法：用HS处理拟南芥根8小时。使用 iTRAQ（相对和绝对定量同量异位标签）技术对根蛋白进行基于定量质谱的蛋白质组学分析。在 HS 处理根与未处理根的 902 个蛋白质家族中进行鉴定和定量，其中 92 个蛋白质具有不同的相对含量。使用 STRING、KEGG、IIS 和 Cytoscape 等生物信息学工具来解释已识别蛋白质之间的生物学功能、通路分析和网络可视化。结果：通过该分析，可以评估所有已识别的蛋白质在功能上分为几个类别，主要是氧化还原稳态、对无机物质的反应、能量代谢、蛋白质合成、细胞运输和分裂。结论：本研究概述了受 HS 生物活性影响最大的代谢途径。糖酵解途径酶的激活和核糖体蛋白的上调表明能量代谢和蛋白质合成受到刺激。参与氧化还原稳态的酶的调节表明活性氧在 HS 诱导反应的信号传导和调节中发挥着关键作用。
Background and Aim: Humic substances (HSs) influence the chemical and physical properties of the soil, and are also known to affect plant physiology and nutrient uptake. This study aimed to elucidate plant metabolic pathways and physiological processes influenced by HS activity. Methods: Arabidopsis roots were treated with HS for 8 h. Quantitative mass spectrometry-based proteomics analysis of root proteins was performed using the iTRAQ (Isobaric Tag for Relative and Absolute Quantification) technique. Out of 902 protein families identified and quantified for HS treated vs. untreated roots, 92 proteins had different relative content. Bioinformatic tools such as STRING, KEGG, IIS and Cytoscape were used to interpret the biological function, pathway analysis and visualization of network amongst the identified proteins. Results: From this analysis it was possible to evaluate that all of the identified proteins were functionally classified into several categories, mainly redox homeostasis, response to inorganic substances, energy metabolism, protein synthesis, cell trafficking, and division. Conclusion: In the present study an overview of the metabolic pathways most modified by HS biological activity is provided. Activation of enzymes of the glycolytic pathway and up regulation of ribosomal protein indicated a stimulation in energy metabolism and protein synthesis. Regulation of the enzymes involved in redox homeostasis suggest a pivotal role of reactive oxygen species in the signaling and modulation of HS-induced responses.