A group of related cDNAs encoding secreted proteins from Hessian fly [Mayetiola destructor (Say)] salivary glands
A group of related cDNAs encoding secreted proteins from Hessian fly [Mayetiola destructor (Say)] salivary glands
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DOI：
10.1111/j.1365-2583.2004.00465.x
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发表时间：
2004-02-01
影响因子：
2.6
通讯作者：
Liu, X
中科院分区：
文献类型：
--
作者：
Chen, MS;Fellers, JP;Liu, X
已从黑森蝇[Mayetiola destructor（Say）]唾液腺中分离并鉴定了一组cDNA。该组中的成员似乎编码在N-末端具有分泌信号肽的蛋白质。成熟的推定蛋白质是小的，碱性蛋白质，计算的分子量范围为8.5至10 kDa，等电点为9.92至10.90。序列分析表明，在编码区内产生氨基酸变化的突变具有很强的选择性。北方印迹分析表明，这些基因只在一龄幼虫中表达，这是一个决定特定黑森蝇生物型和特定小麦品种之间相互作用是否相容的关键阶段。基因组分析表明，相似基因的多个拷贝聚集在染色体2A上的一个短区域内。这是两个无毒基因已被定位的同一臂。
A group of cDNAs has been isolated and characterized from Hessian fly [Mayetiola destructor (Say)] salivary glands. Members in this group appear to encode proteins with secretion signal peptides at the N-terminals. The mature putative proteins are small, basic proteins with calculated molecular weights that ranged from 8.5 to 10 kDa, and isoelectric points from 9.92 to 10.90. Sequence analysis indicated a strong selection for mutations that generate amino acid changes within the coding region. Northern blot analysis revealed that these genes are expressed only in the first instar larvae, a critical stage that determines if the interaction between a specific Hessian fly biotype and a specific wheat cultivar is compatible. Genomic analysis demonstrated that multiple copies of similar genes are clustered within a short region on chromosome 2A. This is the same arm in which two avirulence genes have been mapped.