Bautype: Capsule and Lipopolysaccharide Serotype Prediction for Acinetobacter baumannii Genome
Bautype: Capsule and Lipopolysaccharide Serotype Prediction for Acinetobacter baumannii Genome
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Bautype：鲍曼不动杆菌基因组的荚膜和脂多糖血清型预测
DOI：
10.1097/im9.0000000000000019
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发表时间：
2020-03-01
期刊：
影响因子：
--
通讯作者：
Chen, Huan
中科院分区：
文献类型：
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作者：
Hua, Xiaoting;Liang, Qian;Chen, Huan
鲍曼不动杆菌具有三个多糖合成基因簇。荚膜多糖和脂多糖是许多革兰氏阴性菌的主要毒力决定因子，对A.鲍曼不动杆菌，同时聚-β-1-6-N-乙酰葡糖胺是生物膜形成的关键。目前已开展了多种研究，重点是监测A.鲍曼不动杆菌。然而，目前还没有一种预测A.鲍曼不动杆菌仍然缺乏。在这里，我们提出了Bautype，一个工具，全基因组测序为基础的K和O位点分型在A。鲍曼不动杆菌。使用Bautype，用户可以上传和分析在网络浏览器中组装的基因组，结果以表格或图形格式显示。我们证明了高精度的预测K和O位点多样性使用Bautype分析超过3000 A。鲍曼不动杆菌基因组。利用我们的软件，我们能够分析属于ST 208（3 K和8 O）的593个基因组中的K和O位点的多样性。这些不同的基因座可以作为多药耐药（MDR）菌株亚型的流行病学标记。我们还确定了45个新的O位点类型。我们的研究结果强调了监测胶囊类型和A的LOS的重要性。Bautype是一款针对鲍曼不动杆菌菌株量身定制的临床软件。Bautype可在http://BauType.net上获得。
Acinetobacter baumannii has three clusters of genes for polysaccharide synthesis. Capsular polysaccharides and lipopolysaccharides are major virulence determinants in many Gram-negative bacteria, and play key roles in the overall fitness and virulence in A. baumannii, meanwhile poly-beta-1-6-N-acetylglucosamine was critical for biofilm formation. A variety of studies have been conducted which focused on the surveillance of antimicrobial resistance genes and clones in A. baumannii. However, a tool to perform serotype prediction in A. baumannii is still lacking. Here we present Bautype, a tool for whole genome sequencing-based K and O locus typing in A. baumannii. Using Bautype, users can upload and analyze the genome assembled in a web browser, with the results being displayed in a tabular or graphical format. We demonstrated the high accuracy of predicting K and O locus diversity using Bautype when analyzing more than 3000 A. baumannii genomes. Using our software, we were able to analyze K and O locus diversity among 593 genomes belonging to ST208 (3 K and 8 O). These diverse loci can serve as epidemiological markers for Multi-drug Resistance (MDR) strain subtyping. We also identified 45 novel O locus types. Our findings highlight the importance in monitoring the capsule type and LOS of A. baumannii strains for which Bautype is a tailored clinical software. Bautype is available at http://BauType.net.