Detection of the FK506-FKBP-calcineurin complex by a simple binding assay.
Detection of the FK506-FKBP-calcineurin complex by a simple binding assay.
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通过简单的结合测定检测 FK506-FKBP-钙调磷酸酶复合物。
DOI：
10.1006/bbrc.1993.1570
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
C. Tanaka
中科院分区：
文献类型：
--
作者：
M. Asami;T. Kuno;H. Mukai;C. Tanaka
[~3H]FK506-FK506结合蛋白(FKBP)不与聚乙烯亚胺处理的玻璃纤维过滤器结合，而与钙调神经磷酸酶结合时与过滤器结合。这使我们能够建立一种简单的结合分析方法来检测混合物中的FK506-FKBP-钙调神经磷酸酶复合体，并估计在大鼠组织的可溶性提取物中形成该复合体的FKBP或钙调神经磷酸酶的量。FKBP在大鼠各组织中的摩尔浓度远高于钙调神经磷酸酶，FKBP/钙调神经磷酸酶在不同脑区的摩尔比为13~33，在外周组织中为42~343。这些结果表明，大多数FKBP分子具有与钙调神经磷酸酶无关的生理功能，尽管它们有可能与FK506和钙调神经磷酸酶形成复合体。
[3H]FK506-FK506-binding protein (FKBP) complex does not bind polyethyleneimine-treated glass fiber filters, on the other hand, it binds the filter when it makes a complex with calcineurin. This enabled us to develop a simple binding assay to detect the FK506-FKBP-calcineurin complex in the mixture and to estimate the amount of FKBP or calcineurin which can form the complex in the soluble extracts from rat tissues. The molar concentration of FKBP of every rat tissue is much higher than that of calcineurin, and FKBP/calcineurin molar ratio varies from 13 to 33 in various brain regions, and from 42 to 343 in peripheral tissues. These results suggest that most of the FKBP molecules have physiological roles which are not related to calcineurin, in spite of their potential ability to form the complex with FK506 and calcineurin.