LOCALIZATION OF MUCOSAL MAST-CELLS IN W/WV MICE AFTER RECONSTITUTION WITH BONE-MARROW CELLS OR CULTURED MAST-CELLS, AND ITS RELATION TO THE PROTECTIVE CAPACITY TO STRONGYLOIDES-RATTI INFECTION
LOCALIZATION OF MUCOSAL MAST-CELLS IN W/WV MICE AFTER RECONSTITUTION WITH BONE-MARROW CELLS OR CULTURED MAST-CELLS, AND ITS RELATION TO THE PROTECTIVE CAPACITY TO STRONGYLOIDES-RATTI INFECTION
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DOI：
10.1111/j.1365-3024.1987.tb00524.x
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发表时间：
1987-07-01
影响因子：
2.2
通讯作者：
NAWA, Y
中科院分区：
文献类型：
--
作者：
ABE, T;NAWA, Y
将肥大细胞缺陷的WBB 6 F1（W/Wv）小鼠用骨髓细胞或培养的肥大细胞（BMMC）重建的肠上皮、绒毛固有层和基底固有层中的肥大细胞定位与肥大细胞充足的C57 BL/6或C57 BL/6-bgj/bgj（米色）小鼠在感染鼠类圆线虫后的定位进行比较。在肥大细胞充足的C57 BL/6或beige小鼠中，肠粘膜肥大细胞（MMC）的最大数量超过160个MMC/10个绒毛隐窝单位（VCU），并且超过90%的MMC位于肠上皮中。当W/Wv小鼠与米色小鼠的骨髓细胞重组时，蠕虫排出加快，MMC反应在细胞数量和上皮内定位方面与肥大细胞充足的小鼠相当。另一方面，当W/Wv小鼠用米色小鼠的BMMC重建时，在肠中仅检测到少数供体型MMC。上皮内MMC的比例低于肥大细胞充足的小鼠或骨髓重建的W/Wv小鼠。即使反复注射BMMC也不能完全恢复上皮内MMC的数量到肥大细胞充足小鼠中观察到的水平。由于W/Wv小鼠的肥大细胞生长因子产生活性与肥大细胞充足的小鼠相当，BMMC转移在W/Wv小鼠中恢复保护活性或MMC反应的无效性似乎归因于BMMC的功能不成熟或不活动。
Localization of mast cells in the intestinal epithelium, villous lamina propria and basal lamina propria of mast cell-deficient WBB6F1 (W/Wv) mice reconstituted with either bone marrow cells or with cultured mast cells (BMMC) was compared to that of mast cell-sufficient C57BL/6 or C57BL/6-bgj/bgj (beige) mice after infection with Strongyloides ratti. In mast cell-sufficient C57BL/6 or beige mice, the maximum number of intestinal mucosal mast cells (MMC) was more than 160 MMC/10 villus crypt units (VCU) and more than 90% of MMC were located in the intestinal epithelium. When W/Wv mice were reconstituted with bone marrow cells of beige mice, worm expulsion was hastened and the MMC response became comparable to that of mast cell-sufficient mice in terms of cell numbers and their intra-epithelial localization. On the other hand, when W/Wv mice were reconstituted with BMMC of beige mice, only a few donor type MMC were detected in the intestine. The proportion of intra-epithelial MMC was lower than that of mast cell-sufficient mice or of marrow-reconstituted W/Wv mice. Even repeated injection of BMMC could not fully restore the number of intra-epithelial MMC to the level of that observed in mast cell-sufficient mice. Since mast cell-growth factor-producing activity of W/Wv mice was comparable to that of mast cell-sufficient mice, the ineffectiveness of BMMC-transfer in restoring protective activity or MMC responses in W/Wv mice seems to be attributed to the functional immaturity or inactivity of BMMC.