Immunoglobulin A antibodies against internal HIV-1 proteins neutralize HIV-1 replication inside epithelial cells.
Immunoglobulin A antibodies against internal HIV-1 proteins neutralize HIV-1 replication inside epithelial cells.
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DOI：
10.1016/j.virol.2006.08.006
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发表时间：
2006-12
期刊：
影响因子：
3.7
通讯作者：
A. Wright;Huimin Yan;M. Lamm;YUNG-TSEN Huang
中科院分区：
文献类型：
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作者：
A. Wright;Huimin Yan;M. Lamm;YUNG-TSEN Huang
我们发现，在抗体从基底外侧到顶端表面的转胞吞作用期间，可以发生针对内部病毒蛋白的 IgA 抗体对 HIV 的上皮细胞内中和。用 HIV 前病毒 DNA 转染表达聚合免疫球蛋白受体 (pIgR) 的极化上皮细胞，并将 IgA 添加到基底外侧。通过 HeLa-CD4-LTR/β-Gal 细胞感染和直接 p24 测定评估，针对 Gag 和 RT 的转胞吞 IgA 抗体显着抑制 HIV 复制。与细胞内中和作用一致，共聚焦显微镜证实了内部病毒蛋白及其 IgA 抗体的共定位。因此，至少在极化上皮感染的情况下，抗体介导的中和可能不限于病毒表面抗原。
We show that intraepithelial cell neutralization of HIV by IgA antibodies to internal viral proteins can occur during antibody transcytosis from the basolateral to the apical surface. Polarized epithelial cells expressing the polymeric immunoglobulin receptor (pIgR) were transfected with HIV proviral DNA, and IgA was added to the basolateral side. Transcytosing IgA antibodies against Gag and RT significantly inhibited HIV replication as assessed by infection of HeLa-CD4-LTR/β-Gal cells and direct p24 assay. Consistent with intracellular neutralization, colocalization of the internal virus proteins and their IgA antibodies was demonstrated by confocal microscopy. Thus, at least in the context of infections of polarized epithelia, antibody-mediated neutralization may not be restricted to viral surface antigens.