Degradation of microcystins using immobilized microorganism isolated in an eutrophic lake
Degradation of microcystins using immobilized microorganism isolated in an eutrophic lake
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DOI：
10.1016/j.chemosphere.2006.02.018
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发表时间：
2006-09-01
期刊：
影响因子：
8.8
通讯作者：
Harada, Ken-ichi
中科院分区：
文献类型：
--
作者：
Tsuji, Kiyomi;Asakawa, Miki;Harada, Ken-ichi
本系列研究的最终目的是利用固定化微生物裂解蓝藻并分解微囊藻毒素，建立一种生物去除蓝藻及其有毒产物的方法。为了建立非点区和净水厂的生物去除方法，本研究首先探索了对蓝藻肝毒素微囊藻毒素有活性的细菌。从日本Tsukui湖和Sagami湖的样品中分离出11种活性细菌。在3株具有降解活性的菌株（B-9 ~ B-11）中，菌株B-9的降解活性最强。菌株B-9的16S rDNA序列与Sphingomonas sp. Y2 （AB084247，相似性为99%）相似度最高。菌株B-9 （SC16）在1 d内完全降解了微囊藻毒素- rr和-LR，从而将微生物固定在聚酯树脂上。采用固定化菌株B-9在生物反应器中观察微囊藻毒素- rr的降解情况，24 h后微囊藻毒素- rr（> 90%）被完全降解。将微囊藻毒素- rr定期添加到湖水中，观察24 h后微囊藻毒素- rr在生物反应器中的降解情况，持续72 d。结果表明，在优化条件下，固定化B-9的活性寿命至少为2个月。综上所述，固定化B-9在非点源地区和净水厂的微囊藻毒素处理中是可行的。(c) 2006 Elsevier Ltd.版权所有。
The final purpose of our series of studies is to establish a biological removal method of cyanobacteria and their toxic products using immobilized microorganisms that can lyse cyanobacteria and decompose microcystins. To establish the biological removal method in non-point areas and water purification plants, as the first step, we explored bacteria active against the cyanobacterial hepatotoxin microcystin in the present study. Eleven active bacteria were isolated from samples taken from Lakes Tsukui and Sagami, Japan. Among 3 strains (B-9 to B-11) with degradative activity, strain B-9 exhibited the strongest activity. The 16S rDNA sequence of the strain B-9 showed the highest similarity to that of Sphingomonas sp. Y2 (AB084247, 99% similarity). Microcystins-RR and -LR were completely degraded by strain B-9 (SC16) within 1 d, which led to an immobilized microorganism with a polyester resin. The degradation of microcystin-RR in a bioreactor using the immobilized strain B-9 was observed and microcystin-RR (> 90%) was completely degraded after 24 h. Microcystin-RR was added to the lake water at regular intervals and the degradation after 24 h was observed in the bioreactor over a 72-d period. An over 80% removal efficiency continued for 2 months, showing that the life of the immobilized B-9 in terms of activity was at least 2 months under the optimized conditions. From these results, this immobilized B-9 is feasible for the practical treatment of microcystins in non-point areas and water purification plants. (c) 2006 Elsevier Ltd. All rights reserved.