Modulation of CREB binding protein function by the promyelocytic (PML) oncoprotein suggests a role for nuclear bodies in hormone signaling
Modulation of CREB binding protein function by the promyelocytic (PML) oncoprotein suggests a role for nuclear bodies in hormone signaling
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DOI：
10.1073/pnas.96.6.2627
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发表时间：
1999-03-16
影响因子：
11.1
通讯作者：
Evans, RM
中科院分区：
文献类型：
--
作者：
Doucas, V;Tini, M;Evans, RM
被称为早幼粒细胞（PML）致癌结构域（POD）的球形核小体解聚是急性早幼粒细胞白血病的特征。在这里，我们证明了cAMP增强子结合蛋白（CREB）结合蛋白（CBP）与PML在体外和招募到体内的POD。通过与CBP的结合，野生型PML显著刺激核受体转录活性。这些结果表明，CBP的一部分是通过其与PML的协会区室化的POD，因此表明，PML和其他POD相关的蛋白质可能发挥了出乎意料的广泛的作用，在转录调控和人类疾病的方面。
Disaggregation of the spherical nuclear bodies termed promyelocytic (PML) oncogenic domains (PODs) is a characteristic of acute promyelocytic leukemia. Here, we demonstrate that the cAMP enhancer binding protein (CREB)-binding protein (CBP) associates with PML in vitro and is recruited to the PODs in vivo. Through its association with CBP, wild-type PML dramatically stimulates nuclear receptor transcriptional activity. These results demonstrate that a fraction of CBP is compartmentalized to the POD through its association with PML and thus suggest that PML and other POD-associated proteins may play an unexpectedly broad role in aspects of transcriptional regulation and human disease.