Transparent adult zebrafish as a tool for in vivo transplantation analysis
Transparent adult zebrafish as a tool for in vivo transplantation analysis
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DOI：
10.1016/j.stem.2007.11.002
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发表时间：
2008-02-01
期刊：
影响因子：
23.9
通讯作者：
Zon, Leonard I.
中科院分区：
文献类型：
--
作者：
White, Richard Mark;Sessa, Anna;Zon, Leonard I.
斑马鱼是了解正常和癌症干细胞的有用模型，但由于成鱼的不透明性，分析仅限于胚胎发生。为了解决这个问题，我们创造了一个透明的成年斑马鱼，我们在其中移植了造血干/祖细胞或肿瘤细胞。在造血辐射恢复测定中，GFP标记的骨髓细胞的移植允许从移植后2小时至5周对植入进行引人注目的体内视觉评估。使用FACS分析，透明和野生型鱼具有相等的植入，但这只能在透明受体中可视化。在肿瘤移植模型中，RAS-黑色素瘤细胞的移植允许在短至5天内可视化肿瘤移植、增殖和远处转移，这在野生型受体中直到3至4周才观察到。这种透明的成年斑马鱼是体内干细胞分析的灵敏度和分辨率的理想组合。
The zebrafish is a useful model for understanding normal and cancer stem cells, but analysis has been limited to embryogenesis due to the opacity of the adult fish. To address this, we have created a transparent adult zebrafish in which we transplanted either hematopoietic stem/progenitor cells or tumor cells. In a hematopoiesis radiation recovery assay, transplantation of GFP-labeled marrow cells allowed for striking in vivo visual assessment of engraftment from 2 hr-5 weeks posttransplant. Using FACS analysis, both transparent and wild-type fish had equal engraftment, but this could only be visualized in the transparent recipient. In a tumor engraftment model, transplantation of RAS-melanoma cells allowed for visualization of tumor engraftment, proliferation, and distant metastases in as little as 5 days, which is not seen in wild-type recipients until 3 to 4 weeks. This transparent adult zebrafish serves as the ideal combination of both sensitivity and resolution for in vivo stem cell analyses.