TERMINAL DIFFERENTIATION OF HUMAN PROMYELOCYTIC LEUKEMIA-CELLS INDUCED BY DIMETHYL-SULFOXIDE AND OTHER POLAR COMPOUNDS
TERMINAL DIFFERENTIATION OF HUMAN PROMYELOCYTIC LEUKEMIA-CELLS INDUCED BY DIMETHYL-SULFOXIDE AND OTHER POLAR COMPOUNDS
复制标题
DOI：
10.1073/pnas.75.5.2458
复制
发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
GALLO, RC
中科院分区：
文献类型：
--
作者：
COLLINS, SJ;RUSCETTI, FW;GALLO, RC
最近从一个急性早幼粒细胞白血病患者的外周血白细胞中建立了一个人类白血病细胞系（指定为HL-60）。该细胞系表现出明显的形态和组织化学向髓系分化的承诺。培养的细胞主要是早幼粒细胞，但二甲亚砜的加入可诱导它们分化为髓细胞、变髓细胞和带状和分节中性粒细胞。HL-60培养的150个无性系在二甲亚砜的作用下均表现出相似的形态分化。与形态学未成熟的早幼粒细胞不同，二甲亚砜诱导的成熟细胞表现出功能成熟，如吞噬活性。许多其他化合物先前被证明可以诱导小鼠红细胞白血病（Friend）细胞的红系分化，可以诱导髓系HL-60细胞类似的成熟。HL-60细胞和Friend细胞在这些诱导因子作用下表现出明显的相似性，这表明人类髓系和小鼠红系白血病细胞分化的诱导机制相似。
A human leukemic cell line (designated HL-60) was recently established from peripheral blood leukocytes of a patient with acute promyelocytic leukemia. This cell line displays distinct morphological and histochemical commitment toward myeloid differentiation. The cultured cells are predominantly promyelocytes, but the addition of dimethyl sulfoxide induces them to differentiate into myelocytes, metamyelocytes and banded and segmented neutrophils. All 150 clones developed from the HL-60 culture show similar morphological differentiation in the presence of dimethyl sulfoxide. Unlike the morphologically immature promyelocytes, the dimethyl sulfoxide-induced mature cells exhibit functional maturity as exemplified by phagocytic activity. A number of other compounds previously shown to induce erythroid differentiation of mouse erythroleukemia (Friend) cells can induce analogous maturation of myeloid HL-60 cells. The marked similarity in behavior of HL-60 cells and Friend cells in the presence of these inducing agents suggests that similar molecular mechanisms are involved in the induction of differentiation of these human myeloid and murine erythroid leukemic cells.