Enhanced Charge Transport in Enzyme-Wired Organometallic Block Copolymers for Bioenergy and Biosensors
Enhanced Charge Transport in Enzyme-Wired Organometallic Block Copolymers for Bioenergy and Biosensors
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DOI：
10.1021/ma300155u
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发表时间：
2012-04-10
期刊：
影响因子：
5.5
通讯作者：
Park, Moon Jeong
中科院分区：
文献类型：
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作者：
Lee, Joungphil;Ahn, Hyungmin;Park, Moon Jeong
通过由二茂铁基二甲基硅烷-b-异戊二烯（PFS-PI）组成的交联网络，成功地实现了葡萄糖氧化酶（GOx）在电极表面的连接. PFS-PI的不同纳米级形态，即，双连续结构、纳米线和纳米颗粒已经通过改变分子量和浇铸溶剂得到。在检查GOx集成PFS-PI系统的催化电流响应时，值得注意的是，PFS-PI的形态被发现是决定电子转移效率的关键参数。例如，与其他形态的值相比，使用双连续PFS-PI证实了2-50倍的催化电流密度提高;在70 mM葡萄糖浓度下，葡萄糖氧化的最大催化电流为0.7 mA/cm（2）。通过结构优化制备的电极还发挥了生物传感能力，在血液中葡萄糖的生理浓度下获得了良好的灵敏度。
Wiring of glucose oxidase (GOx) onto electrode surface was successfully achieved by cross-linked networks of organometallic block copolymers comprising electroactive ferrocene moieties and chemically cross-linkable diene groups, poly(ferrocenyldimethylsilane-b-isoprene)s (PFS-PIs). Different nanoscale morphologies of PFS-PIs, i.e., bicontinuous structure, nanowires, and nanoparticles, have been derived by varying molecular weights and casting solvents. Upon examining catalytic current responses of the GOx integrated PFS-PI systems, notably, the morphology of PFS-PI is found out to be a crucial parameter in determining the efficiency of electron transfer. For example, the use of bicontinuous PFS-PI confirms 2-50 times improved catalytic current densities, compared with the values of other morphologies; the maximum catalytic current of glucose oxidation was 0.7 mA/cm(2) at 70 mM glucose concentration. The biosensing ability of the fabricated electrode with structural optimization was also exploited, and good sensitivity is obtained at the physiological concentration of glucose in blood.