Identification of central nervous system neurons that innervate the bladder body, bladder base, or external urethral sphincter of female rats: A transneuronal tracing study using pseudorabies virus
Identification of central nervous system neurons that innervate the bladder body, bladder base, or external urethral sphincter of female rats: A transneuronal tracing study using pseudorabies virus
复制标题
鉴定支配雌性大鼠膀胱体、膀胱基底或尿道外括约肌的中枢神经系统神经元：使用伪狂犬病病毒的跨神经元追踪研究
DOI：
10.1002/(sici)1096-9861(19971229)389:4
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发表时间：
1997
影响因子：
2.5
通讯作者：
L. Marson
中科院分区：
文献类型：
--
作者：
L. Marson
采用跨神经元示踪技术，对雌性大鼠脊髓和脑干中参与排尿的神经元进行了鉴定。将伪狂犬病病毒Bartha K株注射到尿道外括约肌、膀胱底部或膀胱体中。3-5天后，用固定剂灌注大鼠，并通过免疫组织化学鉴定病毒标记的细胞。外尿道括约肌（EUS）注射导致标记的阴部运动神经元的背外侧核L 6。在T13-S1的内侧髓和T13-L2和L5-S1的外侧灰质中发现了假定的脊髓中间神经元。在膀胱基底和膀胱体注射后，在L 6-S1的外侧灰质和内侧索中发现大多数伪狂犬病病毒标记细胞。在中间外侧细胞柱中发现的一些类似于副交感神经节前神经元;推测外侧和内侧灰质中的其余神经元为中间神经元。在T10吻侧发现极少数伪狂犬病病毒标记细胞。在脑干中，在所有注射部位后，在锥体旁髓质网状结构、巴林顿核、中缝大核、中缝苍白核、蓝斑下α部、蓝斑、A5去甲肾上腺素能细胞群和中脑导水管周围灰质腹内侧中发现了transneuronally标记的细胞。在存活时间最长的前脑中也观察到伪狂犬病病毒标记的细胞;一致标记的区域包括外侧下丘脑、室旁核的小细胞区和内侧视前区。这些研究表明，有大量的中枢神经系统神经元的重叠，支配EUS和膀胱的女性。神经学比较杂志389：584-602，1997.© 1997 Wiley利斯公司
Transneuronal tracing techniques were used to identify the putative spinal and brainstem neurons involved in continence and voiding in the female rat. Pseudorabies virus, Bartha's K strain, was injected into either the external urethral sphincter, the bladder base, or the bladder body. After 3–5 days, the rats were perfused with fixative, and virus‐labelled cells were identified by using immunohistochemistry. External urethral sphincter (EUS) injections resulted in labelling of pudendal motoneurons in the dorsolateral nucleus of L6. Putative spinal interneurons were found in the medial cord from T13 to S1 and in the lateral gray of T13–L2 and L5–S1. After both bladder base and bladder body injections, the majority of pseudorabies virus‐labelled cells were found in the lateral gray and medial cord of L6–S1. A number of those found in the intermediolateral cell column resembled the parasympathetic preganglionic neurons; the remaining neurons in the lateral and medial gray were presumed to be interneurons. Very few pseudorabies virus‐labelled cells were found rostral to T10. In the brainstem, transneuronally labelled cells were found in the parapyramidal medullary reticular formation, Barrington's nucleus, raphe magnus, raphe pallidus, subcoeruleus pars alpha, locus coeruleus, the A5 noradrenergic cell group, and ventromedial periaqueductal gray after all injection sites. Pseudorabies virus‐labelled cells were also seen in the forebrain following the longest survival times; areas consistently labelled included the lateral hypothalamus, the parvocellular region of the paraventricular nucleus, and the medial preoptic area. These studies indicate that there is a substantial overlap of central nervous system neurons that innervate the EUS and the bladder in the female. J. Comp. Neurol. 389:584–602, 1997. © 1997 Wiley‐Liss, Inc.