Selenium determination in human plasma lipoprotein fractions by mass spectrometry analysis
Selenium determination in human plasma lipoprotein fractions by mass spectrometry analysis
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DOI：
10.1016/s0162-0134(00)00071-4
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发表时间：
2000-07-15
影响因子：
3.9
通讯作者：
Favier, A
中科院分区：
文献类型：
--
作者：
Ducros, V;Laporte, F;Favier, A
以前的观察表明，脂蛋白可能参与人体硒的运输。为了进一步研究这个问题，硒测定脂蛋白组分分离健康成人的血浆。气相色谱-质谱法使用同位素稀释技术，以确保低量硒的可靠测量。约3%的血浆硒与脂蛋白结合，主要是LDL组分。LDL和HDL的溶剂分馏后，硒的主要部分被回收的蛋白质提取物，这表明它可能被纳入载脂蛋白。Se的确切形式尚未明确确定。考虑到在蛋白质中发现的不同的硒化合物，它被假定为硒代甲硫氨酸，和/或参与硒-硫键。这可以解释为什么LDL中与载脂蛋白B结合的硒的量大约是硒代蛋氨酸随机取代甲硫氨酸的两倍。(C)2000 Elsevier Science S.A. All rights reserved.
Previous observations have suggested that lipoproteins may be involved in the transport of selenium in humans. To further investigate this question, selenium was measured in lipoprotein fractions isolated from plasma of healthy adults. A gas chromatographic-mass spectrometric method using the isotopic dilution technique was developed to ensure a reliable measurement of low amounts of selenium. About 3% of total plasma selenium was bound to lipoproteins, mainly to the LDL fraction. After solvent fractionation of LDL and HDL, the major part of the selenium was recovered in the protein extract, suggesting that it may be incorporated in apolipoproteins. The exact form of Se is not yet clearly established. Considering the different Se compounds found in proteins, it is postulated to be selenomethionine, and/or participating in a selenium-sulphur bond. This could explain why the amount of selenium bound to apolipoprotein B in LDL was about twice that which could be expected from a random substitution of selenomethionine for methionine. (C) 2000 Elsevier Science S.A. All rights reserved.