Increased efficiency of butanol production from spent sulfite liquor by removal of fermentation inhibitors
Increased efficiency of butanol production from spent sulfite liquor by removal of fermentation inhibitors
复制标题
通过去除发酵抑制剂提高亚硫酸废液生产丁醇的效率
DOI：
10.1016/j.jclepro.2020.121356
复制
发表时间：
2020-08-01
影响因子：
11.1
通讯作者：
Chen, Hongzhang
中科院分区：
文献类型：
--
作者：
He, Qin;Chen, Hongzhang
在这项工作中，废亚硫酸盐液（SSL）经两级抑制剂去除后生产丁醇。对SSL进行成分分析，结果表明SSL中含有大量戊糖、己糖、木质素磺酸盐等抑制剂。基于一体化生物炼制理念，提出了纳滤与超滤耦合的三种利用途径。本研究采用纳滤分离糖进行发酵，超滤分离木质素磺酸盐进一步分馏的方法。采用200 da纳滤膜，木素磺酸盐回收率为83.20%，糖损失为19.14%。纳滤渗透开始产生14.32 g/L的乙醇，但在丙酮-丁醇-乙醇（ABE）发酵中只观察到0.25 g/L的丁醇，表明发酵抑制作用仍然存在。通过脱色和离子交换去除二级抑制剂后，与不加抑制剂的商业糖混合物（12.96 g/L丁醇对12.65 g/L丁醇，21.09 g/L ABE）的对照试验相比，SSL的ABE产量有所提高。同时，对SSL发酵抑制剂进行了彻底去除。本研究提供了一种可持续利用废水生产增值产品的方法，可以减少环境污染，节约资金。(C) 2020 Elsevier Ltd.版权所有。
In this work, spent sulfite liquor (SSL) was used to produce butanol after two-levels of inhibitors removal. SSL component analysis was conducted, and the results showed that SSL contained a large proportion of pentose, hexose, lignosulfonates and other inhibitors. Based on the integrated biorefinery concept, three utilization routes were proposed employing the coupling of nanofiltration and ultrafiltration techniques. The route that uses nanofiltration to separate sugars for fermentation and lignosulfonates for further fractionation using ultrafiltration was chosen in this study. A 200-Da nanofiltration membrane was used and resulted in a recovery of 83.20% of lignosulfonates and a loss of 19.14% sugars. Nanofiltration permeate started to produce 14.32 g/L ethanol, but only 0.25 g/L butanol was observed in the acetone-butanol-ethanol (ABE) fermentation, indicating that the fermentation inhibition still existed. After the removal of second-level inhibitors through decolorization and ion exchange, an improved ABE production from SSL was achieved compared with its control test of commercial sugar mixture without inhibitors (12.96 versus 12.65 g/L butanol, and 21.09 versus 20.38 g/L ABE). Meanwhile, the fermentation inhibitors of SSL were thoroughly removed. This study provides a sustainable approach to produce value-added products from wastewater, and can reduce environmental pollution and save capital. (C) 2020 Elsevier Ltd. All rights reserved.