Protein chemical studies of two RNA viruses and their mutants.
Protein chemical studies of two RNA viruses and their mutants.
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两种 RNA 病毒及其突变体的蛋白质化学研究。
DOI：
10.1101/sqb.1966.031.01.024
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发表时间：
1966
期刊：
影响因子：
--
通讯作者：
B. Wittmann
中科院分区：
文献类型：
--
作者：
H. Wittmann;B. Wittmann
在过去的几年中，已经提出了各种方法来阐明遗传编码问题。最成功的方法始于Nirenberg和Matthaei（1961）的观察，即聚U刺激细菌亚细胞系统中聚苯丙氨酸的合成。另一种方法是将特定蛋白质中的氨基酸交换与特定诱变剂在遗传物质中产生的核苷酸改变进行比较。这已经用烟草花叶病毒（TMV）（Wittmann，1959，1960，1962，1964; Tsugita和Fraenkel-Conrat，1960，1962; Funatsu和Fraenkel-Conrat，1964）和色氨酸合成酶（Yanofsky，1963）的突变体完成。
During the past few years various approaches have been made to elucidate the genetic coding problem. The most successful approach began with the observation by Nirenberg and Matthaei (1961) that poly U stimulates the synthesis of polyphenylalanine in a bacterial subcellular system. Another method consists of comparing amino acid exchanges in specific proteins with nucleotide alterations produced in the genetic material by specific mutagens. This has been accomplished with mutants of tobacco mosaic virus (TMV)(Wittmann, 1959, 1960, 1962, 1964; Tsugita and Fraenkel-Conrat, 1960, 1962; Funatsu and Fraenkel-Conrat, 1964) and of tryptophan synthetase (Yanofsky, 1963).