Intraventricular GLP-1 reduces short- but not long-term food intake or body weight in lean and obese rats
Intraventricular GLP-1 reduces short- but not long-term food intake or body weight in lean and obese rats
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DOI：
10.1016/s0006-8993(97)01057-3
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发表时间：
1998-01-01
期刊：
影响因子：
2.9
通讯作者：
Seeley, RJ
中科院分区：
文献类型：
--
作者：
Donahey, JCK;van Dijk, G;Seeley, RJ
当胰高血糖素样肽-1 (7-36) 酰胺 (GLP-1) 注入第三脑室 (IVT) 时，可减少短期食物摄入量。在本实验中，我们评估了 GLP-1 的 NT 给药是否会影响瘦 Long Evans 大鼠和肥胖 Zucker (fa/fa) 大鼠的长期食物摄入和体重。在实验1中，我们重复了观察结果，即给予IVT的10μg GLP-1可减少一小时和两小时的食物摄入量，并将观察结果扩展到脂肪Zucker大鼠。然而，在两种大鼠品系中，这种急性治疗后 24 小时食物摄入量和体重均没有变化。在实验2中，通过微型渗透泵连续IVT输注GLP-1（30μg/天）4天。这种治疗对 Long-Evans 或肥胖 Zucker 大鼠的食物摄入量或体重也没有影响。对照实验证实 GLP-1 在输注期间仍保持生物活性。在最后的实验中，Long-Evans 大鼠在 6 天的时间里每天只能进食两次，每次 2 小时。在每个进入期之前，大鼠接受 15 μg GLP-1 IVT 或载体对照注射。虽然GLP-1在治疗第一天显着减少了食物摄入量，但GLP-1的这种作用很快消失，以致在第二天减少，第三天消失；并且任何时候对体重都没有影响。总的来说，目前的实验并不支持这样的假设：作用于中枢神经系统的 GLP-1 是长期食物摄入和体重的重要调节剂。 (C) 1998 Elsevier Science B.V.
Glucagon-like-peptide-1 (7-36) amide (GLP-1), when infused into the third ventricle (IVT), reduces short-term food intake. In the present experiments, we assessed whether NT administration of GLP-1 could influence long-term food intake and body weight of lean Long Evans rats and of fatty Zucker (fa/fa) rats. In Experiment 1, we replicated the observation that 10 mu g GLP-1, given IVT, reduces one and 2 h food intake, and extended the observation to fatty Zucker rats. However, in both rat strains, 24 h food intake and body weight were unchanged by this acute treatment. In Experiment 2, GLP-1 (30 mu g/day) was infused IVT continuously for 4 days via an osmotic mini-pump. This treatment also had no effect on food intake or body weight in either Long-Evans or fatty Zucker rats. A control experiment verified that the GLP-1 remained biologically active over the duration of the infusion period. In a final experiment, Long-Evans rats were restricted to two 2 h periods of access to food each day for 6 days. Prior to each of these access periods, rats received either 15 mu g of GLP-1 IVT or a vehicle control injection. While GLP-1 significantly reduced food intake on the first day of treatment, this effect of GLP-1 rapidly disappeared such that it was reduced on the second day and absent on the third day; and there was no effect on body weight at any time. Collectively, the present experiments do not support the hypothesis that GLP-1, acting in the CNS, is an important regulator of long-term food intake and body weight. (C) 1998 Elsevier Science B.V.