BASIC FIBROBLAST GROWTH-FACTOR IN RAT SALIVARY-GLANDS
BASIC FIBROBLAST GROWTH-FACTOR IN RAT SALIVARY-GLANDS
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DOI：
10.1007/bf00333701
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发表时间：
1993-09-01
影响因子：
3.6
通讯作者：
ISEKI, S
中科院分区：
文献类型：
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作者：
AMANO, O;YOSHITAKE, Y;ISEKI, S
我们研究了碱性成纤维细胞生长因子（bFGF）的发生和定位在大鼠唾液腺使用的特异性单克隆抗体。结果表明，大鼠唾液腺提取物对牛毛细血管内皮细胞的生长具有显著的刺激活性，其可通过加入抗bFGF的抗体来阻断。碱性成纤维细胞生长因子的浓度在下颌下腺/舌下腺，通过放射免疫测定法测定，约为80%，在大脑中。免疫细胞化学显示，bFGF免疫反应主要位于腮腺、舌下腺和颌下腺的纹管和排泄管内衬的上皮细胞中。此外，强烈的bFGF-免疫反应性观察颗粒曲小管的下颌下腺，主要集中在无颗粒柱细胞，其中躺在大多数弱免疫染色的细胞含有许多顶端分泌颗粒中的少数。在电镜水平上，免疫反应物质弥漫分布在所有免疫反应细胞的细胞质基质和细胞核中，而所有细胞质细胞器包括分泌颗粒中均不存在免疫反应物质。这些结果表明，碱性成纤维细胞生长因子定位于不同的细胞和亚细胞区室从其他生长因子在导管系统的大鼠唾液腺。
We studied the occurrence and localization of basic fibroblast growth factor (bFGF) in rat salivary glands using a specific monoclonal antibody. It was shown that the extract of rat salivary glands has a pronounced stimulatory activity on the growth of bovine capillary endothelial cells, which is blocked by the addition of an antibody against bFGF. The concentration of bFGF in the submandibular/sublingual gland, as determined by radioimmunoassay, was approximately 80% that in the brain. Immunocytochemistry revealed bFGF-immunoreactivity localized primarily in the epithelial cells lining the striated ducts and excretory ducts of the parotid, sublingual and submandibular glands. In addition, intense bFGF-immunoreactivity was observed in the granular convoluted tubule of the submandibular gland, localized predominantly in the agranular pillar cells, which lay in small numbers among the majority of weakly immunostained cells containing many apical secretory granules. At the electron-microscopic level, the immunoreactive material was distributed diffusely in the cytoplasmic matrix and nuclei of all immunoreactive cells, whereas it was absent from all cytoplasmic organelles including the secretory granules. These results indicate that bFGF is localized in different cellular and subcellular compartments from those of other growth factors in the duct system of rat salivary glands.