Oogenesis in the bluefin tuna, Thunnus thynnus L.: a histological and histochemical study.
Oogenesis in the bluefin tuna, Thunnus thynnus L.: a histological and histochemical study.
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蓝鳍金枪鱼 Thunnus thynnus L. 的卵子发生：组织学和组织化学研究。
DOI：
10.14670/hh-17.775
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发表时间：
2002
影响因子：
2
通讯作者：
Emilio Soler Pascual
中科院分区：
文献类型：
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作者：
C. Sarasquete;S. Cárdenas;C. M. D. de González;Emilio Soler Pascual
组织学和组织化学是研究鱼类生殖机制的有效工具，本研究将其应用于鱼类生殖机制的研究。在蓝鳍金枪鱼，Thunnus thymus L.，根据卵母细胞和卵泡发育的形态特征，卵母细胞发育可分为4个主要阶段。初级生长期包括卵原细胞和嗜碱性或前卵黄发生的卵母细胞，分为染色质-核仁和核周期。次级生长期由卵黄发生早期（脂球和卵黄颗粒1）、中期（卵黄颗粒2）和晚期（卵黄颗粒3）的卵黄发生卵母细胞代表。成熟阶段包括卵黄发生后卵母细胞经历成熟过程。在产卵期间，卵巢中既可区分排卵后卵泡（指示产卵），也可区分闭锁卵泡。在蛋黄颗粒中检测到碳水化合物、脂质、蛋白质，特别是富含酪氨酸、色氨酸、胱氨酸、精氨酸、赖氨酸和半胱氨酸的蛋白质，以及磷脂和/或糖脂和中性糖蛋白。此外，在卵黄发生卵母细胞（卵黄颗粒、皮质肺泡、滤泡层和辐射状卵母细胞）中检测到对不同凝集素（ConA、WGA、DBA和UEA）的亲和力，表明存在具有不同糖残基的糖缀合物（甘露糖- Man-和/或葡萄糖-Glc-; N-乙酰基-D-葡糖胺- GlcNAc-和/或唾液酸- NANA-; N-乙酰基-D-半乳糖胺- GalNAc-; L-岩藻糖-Fuc-）。组织化学技术也证明了存在中性脂质小球（空泡石蜡切片）和中性和羧基化的粘液物质在皮质肺泡。用抗卵黄蛋白原（VTG）血清免疫组化，卵黄发生卵母细胞的卵黄颗粒、颗粒状细胞质和滤泡细胞呈阳性反应。卵黄颗粒中也检测到钙，滤泡被膜中也检测到钙。在女性，性腺指数（GSI）逐渐增加，从5月，在早期卵黄，直到6月在中期和晚期卵黄，在那里达到最高值。随后，在整个成熟产卵阶段（7月），GSI逐渐下降，达到最低值在恢复休息期（10月）。
Histology and histochemistry are useful tools to study reproductive mechanisms in fish and they have been applied in this study. In the bluefin tuna, Thunnus thymus L., oocyte development can be divided into 4 principal phases based on the morphological features of developing oocytes and follicles. The primary growth phase includes oogonia and basophilic or previtellogenic oocytes classified as chromatin-nucleolus and perinucleolus stages. The secondary growth phase is represented by vitellogenic oocytes at early (lipid globule and yolk granule 1), mid (yolk granule 2) and late (yolk granule 3) vitellogenesis stages. The maturation phase involves postvitellogenic oocytes undergoing maturation process. During the spawning period, both postovulatory follicles, which indicate spawning, and atretic follicles can be distinguished in the ovary. Carbohydrates, lipids, proteins and specially those rich in tyrosine, tryptophan, cystine, arginine, lysine and cysteine, as well phospholipids and/or glycolipids and neutral glycoproteins were detected in yolk granules. Moreover, affinity for different lectins (ConA, WGA, DBA and UEA) was detected in vitellogenic oocytes (yolk granules, cortical alveoli, follicular layer and zona radiata), indicating the presence of glycoconjugates with different sugar residues (Mannose- Man- and/or Glucose -Glc-; N-acetyl-D-glucosamine- GlcNAc- and/or sialic acid- NANA-; N-acetyl-D-galactosamine- GalNAc-; L-Fucose -Fuc-). Histochemical techniques also demonstrated the presence of neutral lipids in globules (vacuoles in paraffin sections) and neutral and carboxylated mucosubstances in cortical alveoli. By using anti-vitellogenin (VTG) serum, immunohistochemical positive results were demonstrated in yolk granules, granular cytoplasm and follicular cells of vitellogenic oocytes. Calcium was also detected in yolk granules and weakly in follicular envelope. In females, the gonadosomatic index (GSI) increased progressively from May, during early vitellogenesis, until June during mid and late vitellogenesis, where the highest values were reached. Subsequently, throughout the maturation-spawning phases (July), GSI decreased progressively reaching the minimal values during recovering-resting period (October).
K.Murata：“卵膜亚基的低分子量和高分子量前体的同步产生，响应硬骨鱼中雌激素的施用”Gen.Comp.Endocrinol.95。
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