Silencing of homeobox B9 is associated with down-regulation of CD56 and extrathyroidal extension of tumor in papillary thyroid carcinoma
Silencing of homeobox B9 is associated with down-regulation of CD56 and extrathyroidal extension of tumor in papillary thyroid carcinoma
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DOI：
10.1016/j.humpath.2011.09.008
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发表时间：
2012-08-01
期刊：
影响因子：
3.3
通讯作者：
Park, Tae Jun
中科院分区：
文献类型：
--
作者：
Kim, Jang-Hee;Kim, Young Hwa;Park, Tae Jun
甲状腺乳头状癌是甲状腺恶性肿瘤中最常见的类型，而CD56，一种神经细胞粘附分子，在几乎所有的甲状腺乳头状癌病例中通常下调。同源框B9是一种转录因子，属于同源框转录因子基因家族的产物，已知其调节CD 56的转录并促进某些恶性肿瘤的致瘤性和转移。在这项研究中，我们研究了同源框B9的表达和CD56的表达减少的甲状腺乳头状癌的关系，以及它们的表达和临床病理参数之间的关系。因此，我们对72例甲状腺乳头状癌进行了CD56和同源框B9免疫组化染色，并对31例甲状腺乳头状癌进行了Western印迹。CD56蛋白染色显示，65例甲状腺乳头状癌（90.3%）中CD56蛋白减少或缺失，与同源框B9沉默有关（77.8%）（P = .003）。同源框B9表达的缺失与甲状腺外侵犯（P = 0.002）、肿瘤病理分期（P = 0.01）和年龄大于45岁（P = 0.032）相关。然而，CD56染色与临床病理特征无明显关系（P > 0.05）。总之，甲状腺乳头状癌中CD56表达减少与同源框B9相关。此外，同源框B9的沉默在老年人中更常见，并且与甲状腺外延伸和甲状腺乳头状癌的晚期病理阶段有关。皇冠版权所有（C）2012由Elsevier Inc.发布。All rights reserved.
Papillary thyroid carcinoma is the most common type of thyroid malignancy, and CD56, a neural cell adhesion molecule, is typically down-regulated in almost all cases of papillary thyroid carcinoma. Homeobox B9 is a transcription factor, belongs to the products of the homeobox transcription factor gene family, and has been known to regulate transcription of CD56 and to promote tumorigenicity and metastasis in some malignancies. In this study, we investigated the expression and relation of homeobox B9 to reduced expression of CD56 in papillary thyroid carcinomas and also a relationship between their expression and clinicopathologic parameters. Therefore, we performed CD56 and homeobox B9 immunohistochemical staining on 72 papillary thyroid carcinomas and Western blotting on 31 papillary thyroid carcinomas. CD56 protein staining revealed that it was reduced or absent in 65 papillary thyroid carcinomas (90.3%) and was related to silencing of homeobox B9 (77.8%) (P = .003). The loss of homeobox B9 expression was associated with extrathyroidal extension (P = .002), pathologic stage of tumor (P = .01), and age older than 45 years (P = .032). However, the CD56 staining did not reveal any significant relationship with clinicopathologic features (P > .05). In conclusion, reduced expression of CD56 is associated with homeobox B9 in papillary thyroid carcinomas. Furthermore, silencing of homeobox B9 is more common in older age and is linked to extrathyroidal extension and advanced pathologic stage of papillary thyroid carcinoma. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.