Distinct distribution of laminin and its integrin receptors in the pancreas
Distinct distribution of laminin and its integrin receptors in the pancreas
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DOI：
10.1177/002215540205001206
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发表时间：
2002-12-01
影响因子：
3.2
通讯作者：
Harrison, LC
中科院分区：
文献类型：
--
作者：
Jiang, FX;Naselli, G;Harrison, LC
组织功能受细胞外微环境的调节，包括细胞基底膜，其中层粘连蛋白是主要成分。此前，我们发现层粘连蛋白-1促进胰岛细胞的分化和存活。在这里，我们描述了层粘连蛋白及其整合素受体在成人胰腺中的表达模式。虽然它们表达在腺泡细胞和导管上皮的基底膜上，但在胰岛细胞外未检测到层粘连蛋白链。与层粘连蛋白β(1)-链和伽马(1)-链相反，没有检测到层粘连蛋白-1所特有的α(1)-链。层粘连蛋白-10(α(5)β(1)γ(1))在腺泡组织中表达，层粘连蛋白-2(α(2)β(1)伽马(1))和-10在血管中表达。层粘连蛋白连接分子Nidogen-1的分布类似于LNβ(1)和Gamma(1)，而与Nidogen-1竞争的纤毛蛋白-1和-2主要局限于血管。整合素亚单位α(6)和α(3)在腺泡细胞和导管上皮细胞中表达，而在胰岛细胞中缺失。整合素α(6)β(4)仅见于导管细胞，α(6)β(1)见于腺泡和导管细胞，α(3)β(1)见于腺泡、导管和胰岛细胞。这些发现为进一步研究细胞外基质分子及其受体在胰腺功能中的作用奠定了基础。
Tissue function is regulated by the extracellular microenvironment including cell basement membranes, in which laminins are a major component. Previously, we found that laminin-1 promotes differentiation and survival of pancreatic islet cells. Here we characterize the expression pattern of laminins and their integrin receptors in adult pancreas. Although they are expressed in the basement membrane of acinar cells and duct epithelium, no laminin chains examined were detected extracellularly in the pancreatic islets. In contrast to laminin beta(1)- and gamma(1)-chains, the alpha(1)-chain, unique to laminin-1, was not detected. Laminin-10 (alpha(5)beta(1)gamma(1)) was expressed in acinar tissue, whereas laminins-2 (alpha(2)beta(1)gamma(1)) and -10 were expressed in the blood vessels. The laminin connector molecule, nidogen-1, had a distribution similar to that of laminin beta(1) and gamma(1), whereas fibulin-1 and -2, which compete with nidogen-1, were mostly confined to blood vessels. Integrin subunits alpha(6) and alpha(3) were detected in acinar cells and duct epithelial cells, but alpha(6) was absent in islet cells. Integrin alpha(6)beta(4) was detected only in duct cells, alpha(6)beta(1) in both acinar and ductal cells, and alpha(3)beta(1), in acinar, duct, and islet cells. These findings are a basis for further investigation of the role of extracellular matrix molecules and their receptors in pancreas function.